牛溶菌酶和溶血补体活性的改良琼脂平板测定法。
Improved agar plate assays of bovine lysozyme and haemolytic complement activity.
作者信息
Lie O, Syed M, Solbu H
出版信息
Acta Vet Scand. 1986;27(1):23-32. doi: 10.1186/BF03548556.
Abstract
Bovine serum and colostral whey samples were examined for lysozyme and haemolytic complement activity, employing agar plate techniques. The tests were carried out in agarose gel containing Micrococcus lysodeikticus (for lysozyme), and antibody sensitized rabbit erythrocytes (for complement), respectively. The confirmation of lysozyme (E.C.3.2.1.17) — dependent lysis has been presented elsewhere ( 1986), while heat-inaotivation and antibody to C3 were used in the present study to confirm that the haemolytic activity was attributable to the complement cascade. Repeatability and sensitivity of the described tests were found to be superior to those of photometric procedures. Staining and preservation techniques were developed which extended the applicability of the assay, as they made reading of results independent of time and resulted in the plates being very suitable for photography and storage.
摘要
采用琼脂平板技术检测了牛血清和初乳乳清样本中的溶菌酶和溶血补体活性。分别在含有溶壁微球菌（用于检测溶菌酶）和抗体致敏兔红细胞（用于检测补体）的琼脂糖凝胶中进行试验。溶菌酶（E.C.3.2.1.17）依赖性裂解的确认已在其他地方发表（1986年），而在本研究中使用热灭活和抗C3抗体来确认溶血活性归因于补体级联反应。发现所描述试验的重复性和灵敏度优于光度法。开发了染色和保存技术，扩展了该检测方法的适用性，因为它们使结果读取不受时间影响，并使平板非常适合拍照和保存。
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Some properties of the lysozymes in serum and colostrum from cows with high and low lytic power against Micrococcus lysodeikticus.具有高和低溶菌能力的奶牛血清和初乳中溶菌酶的一些特性，该溶菌能力是针对溶壁微球菌而言的。
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Anim Genet. 1986;17(1):39-45. doi: 10.1111/j.1365-2052.1986.tb03186.x.
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