School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
Biosens Bioelectron. 2016 Mar 15;77:894-900. doi: 10.1016/j.bios.2015.10.067. Epub 2015 Oct 26.
Molecularly imprinted polymer (MIP) was prepared on the surface of Au electrode by electrochemically mediated atom transfer radical polymerization (eATRP) with hemoglobin (Hb) both as catalyst and template molecule. Firstly, the condition for eATRP such as the potential, time and Hb concentration were selected and determined to be -0.51 V, 120 min and 20mg/mL, respectively. Further, the electrode modified with MIP (MIP/Au) was carefully examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscope (SEM). Finally, the MIP/Au electrode was used as a biosensor and successfully applied for the determination of Hb by differential pulse voltammetry (DPV) measurement. The results of experiments showed that the proposed biosensor displayed a broader linear range and a lower detection limit for Hb determination when it was compared to those Hb sensors based on MIP. The linear range was from 1.0 × 10(-10) to 1.0 × 10(1)mg/L with a detection limit of 7.8 × 10(-11)mg/L (S/N=3.3). In a word, the work of this paper established a useful way for the preparation and application of biosensor based on protein imprinted polymers.
通过电化学介导原子转移自由基聚合（eATRP），血红蛋白（Hb）既作为催化剂又作为模板分子，在 Au 电极表面制备了分子印迹聚合物（MIP）。首先，选择并确定了 eATRP 的条件，如电位、时间和 Hb 浓度，分别为-0.51 V、120 min 和 20mg/mL。进一步，通过循环伏安法（CV）、电化学阻抗谱（EIS）和扫描电子显微镜（SEM）仔细检查了修饰有 MIP 的电极（MIP/Au）。最后，将 MIP/Au 电极用作生物传感器，并通过差分脉冲伏安法（DPV）测量成功应用于 Hb 的测定。实验结果表明，与基于 MIP 的 Hb 传感器相比，该生物传感器在 Hb 测定中具有更宽的线性范围和更低的检测限。线性范围为 1.0×10(-10)至 1.0×10(1)mg/L，检测限为 7.8×10(-11)mg/L（S/N=3.3）。总之，本文的工作为基于蛋白质印迹聚合物的生物传感器的制备和应用建立了一种有用的方法。
