Aydin Muhammet
Scientific and Technological Research Center, Tekirdağ Namık Kemal University, Tekirdağ, Turkiye.
Turk J Chem. 2025 Apr 4;49(3):371-381. doi: 10.55730/1300-0527.3736. eCollection 2025.
A label-less impedimetric biosensor modified with poly(thiophene)-graft-poly(glycidyl methacrylate) polymer (PThi-g-PGM) was manufactured for p53 protein quantification in human serum samples. In this study, PThi-g-PGM polymer was synthesized, and this polymer matrix was coated using the spin-coating technique on the single-use indium tin oxide (ITO) substrate for anti-p53 antibody immobilization. The anti-p53 antibodies with high affinity for p53 proteins were covalently attached onto the PThi-g-PGM-coated electrode surface. In addition, the affinity of anti-p53 for the p53 protein was monitored at a constant frequency. Under optimized conditions, the impedimetric changes were linearly related to the p53 concentrations, ranging from 0.05 to 15 pg/mL with a low detection limit of 15.9 fg/mL. This biosensor had desirable storage stability, acceptable repeatability, and high reproducibility. Moreover, this impedimetric biosensor could be regenerated through an acidic treatment procedure. Additionally, the suggested biosensor successfully detected the p53 antigen in human serum samples, and good recycling rates (98.41%-109.32%) were found. In summary, the proposed immunosensor may be a powerful tool for the analysis of the p53 protein for early detection of cancer biomarkers.
制备了一种用聚（噻吩）-接枝-聚（甲基丙烯酸缩水甘油酯）聚合物（PThi-g-PGM）修饰的无标记阻抗生物传感器，用于定量检测人血清样本中的p53蛋白。在本研究中，合成了PThi-g-PGM聚合物，并采用旋涂技术将该聚合物基质涂覆在一次性氧化铟锡（ITO）基底上，用于固定抗p53抗体。将对p53蛋白具有高亲和力的抗p53抗体共价连接到PThi-g-PGM涂层电极表面。此外，在恒定频率下监测抗p53对p53蛋白的亲和力。在优化条件下，阻抗变化与p53浓度呈线性关系，范围为0.05至15 pg/mL，检测限低至15.9 fg/mL。该生物传感器具有良好的储存稳定性、可接受的重复性和高再现性。此外，这种阻抗生物传感器可以通过酸性处理程序进行再生。此外，所建议的生物传感器成功检测了人血清样本中的p53抗原，并发现了良好的回收率（98.41%-109.32%）。总之，所提出的免疫传感器可能是分析p53蛋白以早期检测癌症生物标志物的有力工具。
