Marfin Yu S, Aleksakhina E L, Merkushev D A, Rumyantsev E V, Tomilova I K
Ivanovo State University of Chemistry and Technology, Ivanovo, Russia.
Ivanovo State Medical Academy, Ivanovo, Russia.
J Fluoresc. 2016 Jan;26(1):255-61. doi: 10.1007/s10895-015-1707-x. Epub 2015 Oct 31.
Photophysical properties of several BODIPY-based fluorescent dyes were investigated in systems containing blood plasma biomolecules and in model system containing bovine serum albumin in terms of electronic absorption and fluorescence spectroscopy. The interaction between the investigated dyes and protein plasma components changes spectral characteristics of the dyes and leads to bathochromic and hypochromic absorption spectra shifts accompanied by changing of fluorescence intensity. The mechanism of fluorescence changing was defined in the terms of Stern-Volmer theory. It was shown that the static factor of molecular dye-biopolymers complex formation prevails at plasma protein concentration up to 1 g/l, while the higher viscosity range is characterized mainly by nonspecific fluorophore interactions. The increase of fluorescent characteristics of phenyl-substituted BODIPY in the presence of proteins caused by resonance energy transfer and change of physicochemical properties of the molecular environment of the fluorophore was shown for the first time.
通过电子吸收光谱和荧光光谱，研究了几种基于BODIPY的荧光染料在含有血浆生物分子的体系以及含有牛血清白蛋白的模型体系中的光物理性质。所研究的染料与血浆蛋白成分之间的相互作用改变了染料的光谱特性，导致吸收光谱发生红移和减色，并伴随着荧光强度的变化。根据斯特恩-沃尔默理论确定了荧光变化的机制。结果表明，在血浆蛋白浓度高达1 g/l时，分子染料-生物聚合物复合物形成的静态因素占主导，而在较高粘度范围内，主要以非特异性荧光团相互作用为特征。首次表明，在蛋白质存在下，苯基取代的BODIPY荧光特性的增加是由共振能量转移和荧光团分子环境物理化学性质的变化引起的。
