Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, West Bengal, 713 104, India.
Department of Pharmacology, Jessenius Faculty of Medicine in Martin, Comenius University, Sklabinska 26, 036 01, Martin, Slovakia.
Phytother Res. 2016 Jan;30(1):105-11. doi: 10.1002/ptr.5508. Epub 2015 Nov 2.
Zingiber officinale is used for the management of fever, bronchial asthma and cough for thousands of years. While the link to a particular indication has been established in human, the active principle of the formulation remains unknown. Herein, we have investigated a water extracted polysaccharides (WEP) containing fraction from its rhizome. Utilizing a traditional aqueous extraction protocol and using chemical, chromatographic and spectroscopic methods a fraction containing a branched glucan and polygalaturonan in a ratio of 59:1 was characterized. This glucan, which has a molecular mass of 36 kDa, is made up of terminal-, (1,4)- and (1,4,6)-linked α-Glcp residues. Oral administration of WEP in doses of 25 and 50 mg/kg body weight significantly inhibited the number of citric acid-induced cough efforts in guinea pigs. It does not alter the specific airway smooth muscle reactivity significantly. Thus, traditional aqueous extraction method provides molecular entities, which induces antitussive activity without addiction.
姜黄在治疗发热、支气管哮喘和咳嗽方面已经有几千年的历史。虽然其制剂与特定的适应症已经建立了联系，但配方的活性成分仍然未知。在此，我们研究了其根茎中的一种水提多糖（WEP）。利用传统的水提取方案，并使用化学、色谱和光谱方法，表征了一种含有分支葡聚糖和聚半乳糖醛酸聚糖的比例为 59:1 的部分。这种葡聚糖的分子量为 36 kDa，由末端、（1,4）-和（1,4,6）-连接的α-Glcp 残基组成。WEP 以 25 和 50 mg/kg 体重的剂量口服给药，可显著抑制豚鼠柠檬酸诱导的咳嗽次数。它不会显著改变特定气道平滑肌的反应性。因此，传统的水提取方法提供了分子实体，具有镇咳活性而无成瘾性。
