Jana Subrata, Mukherjee Shuvam, Ali Imran, Ray Bimalendu, Ray Sayani
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, West Bengal 713 104, India.
Heliyon. 2020 Nov 13;6(11):e05499. doi: 10.1016/j.heliyon.2020.e05499. eCollection 2020 Nov.
This research was intended to investigate the structural feature, antioxidative activity and interaction with β-lactoglobulin (β-lg) of a polysaccharide () isolated from fruit thru aqueous extraction, protein elimination and chromatographic techniques. Polysaccharide (molecular weight: 21,000 g mol) was constituted of arabinose, galactose, glucose, and rhamnose in a 50:24:20:6 M ratio alongside 9% (w/w) galacturonic acid. It encompassed a petite backbone entailing galacturonopyranosyl and rhamnopyranosyl units substituted with sizable side chains comprising of arabinofuranosyl, galactopyranosyl and esterified coumaric acid (CA) residues. Various series of oligosaccharides including (i) GalAc, (ii) AraAc, (iii) GalAraAc, (iv) GalAraAc, (v) GalAraAc and (vi) GalAraCAAc and GalAraCAAc epitomizing polysaccharide structure were generated and characterised. Fraction exhibited dose-dependent antioxidant activity and possessed a strong β-lactoglobulin binding capability. Accordingly, fruit offers an antioxidative polysaccharide having novel structure that can associate with β-lg and, hence, useful in formulating novel food possessing adjustable composition.
本研究旨在通过水提取、蛋白质去除和色谱技术，研究从[水果名称]果实中分离得到的一种多糖（[多糖名称]）的结构特征、抗氧化活性及其与β-乳球蛋白（β-lg）的相互作用。多糖[多糖名称]（分子量：21,000 g/mol）由阿拉伯糖、半乳糖、葡萄糖和鼠李糖以50:24:20:6的摩尔比组成，同时含有9%（w/w）的半乳糖醛酸。它包含一个由吡喃半乳糖醛酸基和吡喃鼠李糖基单元组成的小主链，这些单元被由阿拉伯呋喃糖基、吡喃半乳糖基和酯化香豆酸（CA）残基组成的大侧链取代。生成并表征了包括（i）GalAc、（ii）AraAc、（iii）GalAraAc、（iv）GalAraAc、（v）GalAraAc以及（vi）GalAraCAAc和GalAraCAAc等代表多糖结构的各种系列寡糖。组分[具体组分]表现出剂量依赖性抗氧化活性，并具有很强的β-乳球蛋白结合能力。因此，[水果名称]果实提供了一种具有新颖结构的抗氧化多糖，该多糖可与β-lg结合，从而可用于配制具有可调节成分的新型食品。
