天麻对人胚胎神经干细胞增殖与分化的影响。
Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells.
作者信息
Baral Samrat, Pariyar Ramesh, Yoon Chi-Su, Kim Dong-Cheol, Yun Jong-Min, Jang Seok O, Kim Sung Yeon, Oh Hyuncheol, Kim Youn-Chul, Seo Jungwon
机构信息
From the BK21 Plus Team, South Korea; Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea.
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea.
出版信息
Asian Pac J Trop Med. 2015 Oct;8(10):792-7. doi: 10.1016/j.apjtm.2015.09.004. Epub 2015 Sep 25.
OBJECTIVE
To investigate the effects of Gastrodiae rhizoma, a dried root of Gastrodia elata Blume, on proliferation and differentiation of human NSCs derived from embryonic stem cells.
METHODS
A 70% ethanol extract of Gastrodiae rhizoma (EEGR) was estimated with 4-hydroxybenzyl alcohol as a representative constituent by HPLC.
RESULTS
MTT assay showed that the treatment with EEGR increased the viability of NSCs in growth media. Compared to control, EEGR increased the number of dendrites and denritic spines extended from a differentiated NSC. Whereas EEGR decreased the mRNA expression of Nestin, it increased that of Tuj1 and MAP2 in NSCs grown in differentiation media. Immunocytochemical analysis using confocal microscopy also revealed the increased expression of MAP2 in dendrites of EEGR-treated NSCs. Furthermore, EEGR decreased mRNA expression of Sox2 in NSCs grown even in growth media.
CONCLUSIONS
In conclusion, our study demonstrates for the first time that EEGR induced proliferation and neuronal differentiation of NSCs, suggesting its potential benefits on NSC-based therapies and neuroregeneration in various neurodegenerative diseases and brain injuries.
目的
研究天麻（天麻干燥根）对源自胚胎干细胞的人神经干细胞增殖和分化的影响。
方法
以4-羟基苄醇为代表性成分，采用高效液相色谱法测定天麻70%乙醇提取物（EEGR）。
结果
MTT法显示，EEGR处理可提高生长培养基中神经干细胞的活力。与对照组相比，EEGR增加了分化神经干细胞伸出的树突和树突棘的数量。虽然EEGR降低了巢蛋白的mRNA表达，但在分化培养基中生长的神经干细胞中，它增加了Tuj1和MAP2的mRNA表达。使用共聚焦显微镜的免疫细胞化学分析也显示，EEGR处理的神经干细胞树突中MAP2的表达增加。此外，即使在生长培养基中生长的神经干细胞中，EEGR也降低了Sox2的mRNA表达。
结论
总之，我们的研究首次证明EEGR可诱导神经干细胞增殖和神经元分化，表明其在基于神经干细胞的治疗以及各种神经退行性疾病和脑损伤的神经再生方面具有潜在益处。
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