Uesaka Karin, Maezawa Masaki, Inokuma Hisashi
Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
J Vet Med Sci. 2016 Mar;78(3):463-5. doi: 10.1292/jvms.15-0392. Epub 2015 Oct 31.
A serological survey of Borrelia infection of dogs was performed in Sapporo, Japan, where Borrelia garinii infection in dogs was detected in 2011. A total of 314 serum samples were collected from dogs that visited three animal hospitals in Sapporo from 2012 to 2014. The two-step evaluation method, involving screening ELISA followed by Western blot analysis, was used to detect antibodies against Borrelia species. A total of 34 samples were positive by ELISA. Among those 34 samples, 32 were positive for Borrelia spp. by Western blot. These findings suggest that the 32 dogs (10.2%) generated antibodies against Borrelia burgdorferi sensu lato, such as B. garinii or B. afzelii. Antibody positivity was 7.6% and 13.3% for dogs living in urban and rural areas, respectively. Dogs with a history of tick infestation showed a positive rate of 16.7%, which was higher, although not significantly, than the 6.7% among dogs without a history.
在日本札幌进行了一项犬类疏螺旋体感染的血清学调查，2011年在当地犬类中检测到了加氏疏螺旋体感染。2012年至2014年期间，从札幌三家动物医院就诊的犬类中总共采集了314份血清样本。采用两步评估法，即先进行筛选酶联免疫吸附测定（ELISA），然后进行蛋白质印迹分析，以检测针对疏螺旋体属的抗体。ELISA检测共有34份样本呈阳性。在这34份样本中，蛋白质印迹法检测有32份对疏螺旋体属呈阳性。这些结果表明，这32只犬（10.2%）产生了针对广义伯氏疏螺旋体的抗体，如加氏疏螺旋体或阿氏疏螺旋体。城市和农村地区犬类的抗体阳性率分别为7.6%和13.3%。有蜱虫寄生史的犬类阳性率为16.7%，虽高于无蜱虫寄生史犬类的6.7%，但差异不显著。
