诱导型一氧化氮合酶和p53在口腔鳞状细胞癌中的表达及相关性
The Expression and Correlation of iNOS and p53 in Oral Squamous Cell Carcinoma.
作者信息
Yang Lan, Wang Youyuan, Guo Lvhua, Wang Liping, Chen Weiliang, Shi Bin
机构信息
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, China ; Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510120, China.
出版信息
Biomed Res Int. 2015;2015:637853. doi: 10.1155/2015/637853. Epub 2015 Oct 7.
Oral squamous cell carcinoma (OSCC) is the most prevalent form of oral cancer. Inducible nitric oxide synthase (iNOS) and p53 are associated with a variety of human cancers, but their expression and interaction in OSCC have not been fully explored. In this study, we investigated the expression of iNOS and p53 in OSCC and their correlation with tumor development and prognosis. In addition, we explored the interaction of iNOS and p53 in OSCC. The expression of iNOS and p53 in OSCC was investigated using immunohistochemical method and their interaction was studied using RNAi technique. Our results showed that the expression of both iNOS and p53 was significantly correlated with tumor stages and pathological grade of OSCC (P < 0.05). In contrast, there was no correlation between iNOS and p53 expression and lymph node metastasis (P < 0.05). The OSCC survival rate was negatively associated with iNOS expression, but not with p53. A significant increase in the expression of the p53 was observed when iNOS expression was knocked down. The immunoexpression of iNOS is correlated with tumorigenesis and prognosis of OSCC and may serve as a prognostic marker.
口腔鳞状细胞癌（OSCC）是最常见的口腔癌形式。诱导型一氧化氮合酶（iNOS）和p53与多种人类癌症相关，但它们在OSCC中的表达及相互作用尚未得到充分研究。在本研究中，我们调查了iNOS和p53在OSCC中的表达及其与肿瘤发展和预后的相关性。此外，我们还探究了iNOS和p53在OSCC中的相互作用。采用免疫组织化学方法研究iNOS和p53在OSCC中的表达，并运用RNA干扰技术研究它们的相互作用。我们的结果显示，iNOS和p53的表达均与OSCC的肿瘤分期和病理分级显著相关（P < 0.05）。相比之下，iNOS和p53的表达与淋巴结转移之间无相关性（P < 0.05）。OSCC生存率与iNOS表达呈负相关，但与p53无关。当iNOS表达被敲低时，观察到p53表达显著增加。iNOS的免疫表达与OSCC的肿瘤发生及预后相关，可能作为一种预后标志物。
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