Badugu Ramachandram, Szmacinski Henryk, Ray Krishanu, Descrovi Emiliano, Ricciardi Serena, Zhang Douguo, Chen Junxue, Huo Yiping, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 W. Lombard Street, Baltimore, MD 21201, USA.
Department of Applied Science and Technology, Polytechnic University of Turin, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.
ACS Photonics. 2015 Jun 26;2(7):810-815. doi: 10.1021/acsphotonics.5b00219.
We report a metal-dielectric planar structure which provides high efficiency coupling of fluorescence at distances over 100 nm away from the metal surface. This hybrid metal-dielectric waveguide (MDW) consists of a continuous metal film coated with a dielectric layer. We observed efficient long-range coupling of Rhodamine B on top of a 130 nm layer of silica resulting in a narrow angular distribution of the emission. The high efficiency radiation through the Ag film appears to be due to coupling of the fluorophore to an optical waveguide mode with a long propagation length and a narrow resonance. The results were consistent with simulations. These multi-layer structures can be made using vapor deposition and/or spin coating and the silica surface can be used for conjugation to biomolecules and surface-selective detection. This simple hybrid metal-dielectric structures provides new opportunities for fluorescence sensing, genomics, proteomics and diagnostics.
我们报道了一种金属-电介质平面结构，该结构能在距离金属表面超过100纳米的距离上实现高效的荧光耦合。这种混合金属-电介质波导（MDW）由涂有介电层的连续金属膜组成。我们观察到在130纳米厚的二氧化硅层顶部的罗丹明B实现了高效的远程耦合，从而导致发射的角度分布变窄。通过银膜的高效辐射似乎是由于荧光团与具有长传播长度和窄共振的光波导模式耦合所致。结果与模拟结果一致。这些多层结构可以通过气相沉积和/或旋涂制备，并且二氧化硅表面可用于与生物分子共轭和表面选择性检测。这种简单的混合金属-电介质结构为荧光传感、基因组学、蛋白质组学和诊断提供了新的机会。
