Ou Min, Huang Jin, Yang Xiaohai, Quan Ke, Yang Yanjing, Xie Nuli, Wang Kemin
State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province , Hunan University , Changsha , P. R. China . Email:
Chem Sci. 2017 Jan 1;8(1):668-673. doi: 10.1039/c6sc03162e. Epub 2016 Sep 5.
The donor donor-acceptor (DD-A) FRET model has proven to have a higher FRET efficiency than donor-acceptor acceptor (D-AA), donor-acceptor (D-A), and donor donor-acceptor acceptor (DD-AA) FRET models. The in-tube and in-cell experiments clearly demonstrate that the "DD-A" FRET binary probes can indeed increase the FRET efficiency and provide higher imaging contrast, which is about one order of magnitude higher than the ordinary "D-A" model. Furthermore, MnO nanosheets were employed to deliver these probes into living cells for intracellular TK1 mRNA detection because they can adsorb ssDNA probes, penetrate across the cell membrane and be reduced to Mn ions by intracellular GSH. The results indicated that the MnO nanosheet mediated "DD-A" FRET binary probes are capable of sensitive and selective sensing gene expression and chemical-stimuli changes in gene expression levels in cancer cells. We believe that the MnO nanosheet mediated "DD-A" FRET binary probes have the potential as a simple but powerful tool for basic research and clinical diagnosis.
供体-供体-受体（DD-A）荧光共振能量转移（FRET）模型已被证明比供体-受体-受体（D-AA）、供体-受体（D-A）和供体-供体-受体-受体（DD-AA）FRET模型具有更高的FRET效率。管内和细胞内实验清楚地表明，“DD-A”FRET二元探针确实可以提高FRET效率并提供更高的成像对比度，这比普通的“D-A”模型高约一个数量级。此外，MnO纳米片被用于将这些探针递送至活细胞中以进行细胞内胸苷激酶1（TK1）mRNA检测，因为它们可以吸附单链DNA探针，穿透细胞膜并被细胞内的谷胱甘肽（GSH）还原为锰离子。结果表明，MnO纳米片介导的“DD-A”FRET二元探针能够灵敏且选择性地检测癌细胞中的基因表达以及基因表达水平的化学刺激变化。我们相信，MnO纳米片介导的“DD-A”FRET二元探针有潜力成为基础研究和临床诊断中一种简单而强大的工具。
