Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Materials and Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
Bioresour Technol. 2016 Jan;200:541-7. doi: 10.1016/j.biortech.2015.10.075. Epub 2015 Oct 28.
Oil palm mesocarp fiber was subjected to hydrothermal pretreatment under isothermal and non-isothermal conditions. The pretreated slurries were separated by filtration, pretreated liquids and solids were characterized. An enzymatic digestibility study was performed for both pretreated slurries and solids to understand the effect of soluble inhibitors generated during the pretreatment process. The highest glucose yield obtained from pretreated slurries was 70.1%, and gradually decreased with higher pretreatment severities. The highest glucose yield obtained in pretreated solids was 100%, after pretreatment at 210°C for 20min. In order to study the inhibitory effects of compounds generated during pretreatment with cellulase, technical grade solutions that mimic the pretreated liquid were prepared and their effect on Acremonium cellulase activity was monitored using Avicel. Xylo-oligomers and tannic acid were identified as powerful inhibitors of Acremonium cellulase, and the lowest hydrolysis rate of Avicel of 0.18g/g-glucose released/L/h was obtained from tannic acid.
油棕中果皮纤维在等温及非等温条件下进行水热预处理。预处理后的浆料通过过滤进行分离，对预处理后的液体和固体进行了特性分析。对两种预处理后的浆料和固体进行了酶解研究，以了解在预处理过程中生成的可溶性抑制剂的影响。从预处理后的浆料中获得的最高葡萄糖得率为 70.1%，随着预处理强度的增加逐渐降低。在 210°C 下预处理 20min 后，从预处理后的固体中获得的最高葡萄糖得率为 100%。为了研究用纤维素酶预处理过程中生成的化合物的抑制作用，制备了模拟预处理液的工业级溶液，并使用 Avicel 监测其对节杆菌纤维素酶活性的影响。木二糖低聚物和鞣酸被鉴定为节杆菌纤维素酶的强抑制剂，从鞣酸中获得的 Avicel 水解率最低，为 0.18g/g-葡萄糖释放/L/h。
