Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400 UPM, Malaysia.
Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Serdang, Selangor 43400 UPM, Malaysia.
Molecules. 2018 May 30;23(6):1310. doi: 10.3390/molecules23061310.
The present work aimed to investigate the pretreatment of oil palm mesocarp fiber (OPMF) in subcritical H₂O-CO₂ at a temperature range from 150⁻200 °C and 20⁻180 min with CO₂ pressure from 3⁻5 MPa. The pretreated solids and liquids from this process were separated by filtration and characterized. Xylooligosaccharides (XOs), sugar monomers, acids, furans and phenols in the pretreated liquids were analyzed by using HPLC. XOs with a degree of polymerization X2⁻X4 comprising xylobiose, xylotriose, xylotetraose were analyzed by using HPAEC-PAD. Enzymatic hydrolysis was performed on cellulose-rich pretreated solids to observe xylose and glucose production. An optimal condition for XOs production was achieved at 180 °C, 60 min, 3 MPa and the highest XOs obtained was 81.60 mg/g which corresponded to 36.59% of XOs yield from total xylan of OPMF. The highest xylose and glucose yields obtained from pretreated solids were 29.96% and 84.65%, respectively at cellulase loading of 10 FPU/g-substrate.
本工作旨在研究在 150⁻200°C 和 20⁻180 min 的温度范围内，CO₂压力为 3⁻5 MPa 的亚临界 H₂O-CO₂中对油棕中果皮纤维（OPMF）进行预处理。通过过滤将该过程中的预处理固体和液体分离并进行了表征。用高效液相色谱法（HPLC）分析了预处理液体中的低聚木糖（XOs）、糖单体、酸、呋喃和酚类物质。用 HPAEC-PAD 分析了聚合度 X2⁻X4 的 XOs，包括木二糖、木三糖、木四糖。对富含纤维素的预处理固体进行酶水解，以观察木糖和葡萄糖的生成情况。在 180°C、60min、3MPa 的最佳条件下，获得了最高的 81.60mg/g XOs，这相当于 OPMF 中总木聚糖的 36.59%的 XOs 产率。在纤维素酶用量为 10 FPU/g-底物时，预处理固体的最高木糖和葡萄糖产率分别为 29.96%和 84.65%。
