Tang Juan, Lin Jing, Yang Yuwen, Chen Tianzi, Ling Xitie, Zhang Baolong, Chang Youhong
Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Gene. 2016 Jan 15;576(1 Pt 3):492-8. doi: 10.1016/j.gene.2015.10.068. Epub 2015 Oct 31.
Verticillium wilt is a soil borne disease that can cause devastating losses to the production of many economically important crops. A Ve1 homologous gene responding to Verticillium dahliae infection was identified in Vitis vinifera cv. "HeiFeng" by semi-quantitative reverse transcription polymerase chain reaction and was designated as VvVe. The overexpression of VvVe in transgenic Nicotiana benthamiana plants significantly enhanced the resistance to isolate V991 of V. dahliae when compared with the wild type plants. The expressions of defense-related genes including the salicylic acid regulated gene pathogen-related 1 (PR1) but not PR2, the ethylene- and jasmonic acid-regulated genes ethylene response factor 1 (ERF1) and lipoxygenase (LOX) were significantly increased due to over expression of VvVe. And greater accumulation of active oxygen, callose and phenylalanine-ammonia lyase were observed in the leaves of transgenic VvVe tobacco plants than the wild type when under infection by V. dahliae. Moreover, the hypersensitive response mimicking cell death was exclusively occurred in the transgenic VvVe tobacco plants but not in the wild type. Taken together, the VvVe gene is a Ve1 like gene which involves in the signal cascade of salicylic acid, jasmonate, and ethylene defense pathways and enhances defense response to V. dahliae infection in the transgenic tobacco.
黄萎病是一种土传病害，会给许多经济作物的生产造成毁灭性损失。通过半定量逆转录聚合酶链反应，在酿酒葡萄品种“黑丰”中鉴定出一个响应大丽轮枝菌感染的Ve1同源基因，并将其命名为VvVe。与野生型植株相比，VvVe在转基因本氏烟草植株中的过表达显著增强了对大丽轮枝菌V991分离株的抗性。由于VvVe的过表达，包括水杨酸调节基因病程相关蛋白1（PR1）而非PR2、乙烯和茉莉酸调节基因乙烯响应因子1（ERF1）和脂氧合酶（LOX）在内的防御相关基因的表达显著增加。在大丽轮枝菌感染下，转基因VvVe烟草植株叶片中活性氧、胼胝质和苯丙氨酸解氨酶的积累量比野生型更多。此外，模拟细胞死亡的过敏反应仅在转基因VvVe烟草植株中出现，野生型中未出现。综上所述，VvVe基因是一个类似Ve1的基因，参与水杨酸、茉莉酸和乙烯防御途径的信号级联反应，并增强转基因烟草对大丽轮枝菌感染的防御反应。
