Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Sci Rep. 2018 Oct 18;8(1):15404. doi: 10.1038/s41598-018-33743-x.
Verticillium wilt caused by the soil-borne fungus Verticillium dahliae is a common, devastating plant vascular disease notorious for causing economic losses. Despite considerable research on plant resistance genes, there has been little progress in modeling the effects of this fungus owing to its complicated pathogenesis. Here, we analyzed the transcriptional and metabolic responses of Arabidopsis thaliana to V. dahliae inoculation by Illumina-based RNA sequencing (RNA-seq) and nuclear magnetic resonance (NMR) spectroscopy. We identified 13,916 differentially expressed genes (DEGs) in infected compared with mock-treated plants. Gene ontology analysis yielded 11,055 annotated DEGs, including 2,308 for response to stress and 2,234 for response to abiotic or biotic stimulus. Pathway classification revealed involvement of the metabolic, biosynthesis of secondary metabolites, plant-pathogen interaction, and plant hormone signal transduction pathways. In addition, 401 transcription factors, mainly in the MYB, bHLH, AP2-EREBP, NAC, and WRKY families, were up- or downregulated. NMR analysis found decreased tyrosine, asparagine, glutamate, glutamine, and arginine and increased alanine and threonine levels following inoculation, along with a significant increase in the glucosinolate sinigrin and a decrease in the flavonoid quercetin glycoside. Our data reveal corresponding changes in the global transcriptomic and metabolic profiles that provide insights into the complex gene-regulatory networks mediating the plant's response to V. dahliae infection.
由土壤传播真菌黄萎轮枝菌引起的黄萎病是一种常见的、具有破坏性的植物维管束疾病，以引起经济损失而闻名。尽管对植物抗性基因进行了大量研究，但由于其复杂的发病机制，在模拟该真菌的影响方面几乎没有取得进展。在这里，我们通过基于 Illumina 的 RNA 测序（RNA-seq）和核磁共振（NMR）光谱分析了拟南芥对黄萎轮枝菌接种的转录组和代谢组响应。与模拟处理的植物相比，我们在感染的植物中鉴定出了 13916 个差异表达基因（DEGs）。基因本体论分析产生了 11055 个注释的 DEGs，包括 2308 个对胁迫的反应和 2234 个对非生物或生物刺激的反应。途径分类表明代谢途径、次生代谢物生物合成、植物-病原体相互作用和植物激素信号转导途径的参与。此外，上调或下调了 401 个转录因子，主要在 MYB、bHLH、AP2-EREBP、NAC 和 WRKY 家族。NMR 分析发现接种后酪氨酸、天冬酰胺、谷氨酸、谷氨酰胺和精氨酸水平降低，丙氨酸和苏氨酸水平升高，同时硫代葡萄糖苷黑芥子苷显著增加，类黄酮槲皮苷糖苷减少。我们的数据揭示了全局转录组和代谢谱的相应变化，为阐明介导植物对黄萎轮枝菌感染反应的复杂基因调控网络提供了线索。
