Zhang Wanqi, Hu Jianjun, Yan Shilei, Huang Yaojie, Xu Jianping, Huang Zhongwu, Zheng Maoliang, Meng Ziyan, Li Yuanyuan, Wang Na, Wang Qingqing
Bing Du Xue Bao. 2015 Jul;31(4):370-8.
To study the complete genomic sequence, genomic characteristics, and genetic variation of the bovine papillomavirus 2 genotype (BPV-2) Aks-01 strain at the molecular level, genotyping of this strain from the skin samples of cows in southern Xinjiang (China) was first detected by the polymerase chain reaction with FAP59/FAP64 primers. Based on the complete genome of the BPV-2 reference strain, specific primers and sequencing primers were designed, and the complete genome of the Aks-01 strain amplified and sequenced. Sequence analyses showed that genotyping of the Aks-01 strain belonged to BPV-2. The Aks-01 strain had the structural characteristics of BPV-2. The 7944-bp full-length genomic sequence of the Aks-01 strain was compiled using DNAStar™. The sequence of the Aks-01 strain had 98% similarity to the reference strain from GenBank. The Aks-01 strain was most closely related to BPV-1 and BPV-13. BPV-2, BPV-1 and BPV-13 were grouped within the genus Deltapapillomavirus. The Aks-01 strain is the first BPV-2 strain reported in southern Xinjiang.
为在分子水平上研究牛乳头瘤病毒2型（BPV-2）Aks-01株的全基因组序列、基因组特征及遗传变异，首先采用FAP59/FAP64引物通过聚合酶链反应对来自中国新疆南部奶牛皮肤样本中的该毒株进行基因分型检测。基于BPV-2参考毒株的全基因组，设计了特异性引物和测序引物，对Aks-01株的全基因组进行扩增和测序。序列分析表明，Aks-01株的基因分型属于BPV-2。Aks-01株具有BPV-2的结构特征。使用DNAStar™软件编辑了Aks-01株7944bp的全长基因组序列。Aks-01株的序列与GenBank中的参考毒株具有98%的相似性。Aks-01株与BPV-1和BPV-13的亲缘关系最为密切。BPV-2、BPV-1和BPV-13被归为δ乳头瘤病毒属。Aks-01株是新疆南部报道的首例BPV-2毒株。
