Daudt Cíntia, Silva Flavio R C da, Cibulski Samuel P, Weber Matheus N, Mayer Fabiana Q, Varela Ana Paula M, Roehe Paulo M, Canal Cláudio W
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Laboratório de Biologia Molecular, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, RS, Brasil.
Mem Inst Oswaldo Cruz. 2016 Apr;111(4):277-9. doi: 10.1590/0074-02760160047.
The complete genome sequence of bovine papillomavirus 2 (BPV2) from Brazilian Amazon Region was determined using multiple-primed rolling circle amplification followed by Illumina sequencing. The genome is 7,947 bp long, with 45.9% GC content. It encodes seven early (E1, E2,E4, E5, E6,E7, and E8) and two late (L1 and L2) genes. The complete genome of a BPV2 can help in future studies since this BPV type is highly reported worldwide although the lack of complete genome sequences available.
利用多重引物滚环扩增技术,随后进行Illumina测序,测定了来自巴西亚马逊地区的牛乳头瘤病毒2型(BPV2)的全基因组序列。该基因组长度为7947 bp,GC含量为45.9%。它编码7个早期基因(E1、E2、E4、E5、E6、E7和E8)和2个晚期基因(L1和L2)。尽管缺乏可用的全基因组序列,但这种BPV类型在全球范围内有大量报道,因此BPV2的全基因组将有助于未来的研究。