Tomita Yoshimi, Literak Ivan, Ogawa Tomoko, Jin Zhongri, Shirasawa Hiroshi
Department of Molecular Virology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuou-ku, Chiba 260-8670, Japan.
Virus Genes. 2007 Oct;35(2):243-9. doi: 10.1007/s11262-006-0055-y. Epub 2007 Jan 30.
The DNA of bovine papillomavirus (BPV) type 8 was extracted from papillomas on cattle kept in Japan, and DNA of bovine papillomavirus BPV-8-EB was extracted from a European bison (Bison bonasus) born in Italy and released into the wild in Slovakia. The DNA genomes of these BPVs were amplified using multiply primed rolling circle amplification and polymerase chain reaction, then characterized by direct sequencing method. The BPV-8 and BPV-8-EB genomes consisted of 7,791 base pairs (bp) and 7,773 bp, respectively (GenBank accession numbers DQ098913 and DQ098917). The nucleotide sequence similarity of these BPVs indicated that BPV-8-EB was a variant of BPV-8. In the genome of BPV-8-EB, one nucleotide substitution was found in the E2 and E5 open reading frame (ORF) and upstream regulatory region (URR), and a short deletion and addition were found in the URR. The high similarity of sequences between the BPV-8 to BPV-5 in total genome (70%) and L1 ORF (75%) as well as a phylogenetic analysis were the bases for classifying BPV-8 in the genus Epsilon papillomavirus. The BPV-8 E6 and E7 ORFs/proteins also showed some characteristic features of genus Epsilon papillomavirus. However, BPV-8 contained E4 ORF, which was not found in BPV-5. In addition, the secondary structure of E5 proteins of BPV-5 and BPV-8 suggested that these proteins may have cell-transforming ability.
牛乳头瘤病毒8型（BPV）的DNA是从日本饲养的牛的乳头瘤中提取的，而牛乳头瘤病毒BPV - 8 - EB的DNA是从出生于意大利并放归斯洛伐克野外的一头欧洲野牛（欧洲野牛）中提取的。这些BPV的DNA基因组通过多重引物滚环扩增和聚合酶链反应进行扩增，然后通过直接测序法进行特征分析。BPV - 8和BPV - 8 - EB基因组分别由7791个碱基对（bp）和7773个bp组成（GenBank登录号分别为DQ098913和DQ098917）。这些BPV的核苷酸序列相似性表明BPV - 8 - EB是BPV - 8的一个变体。在BPV - 8 - EB的基因组中，在E2和E5开放阅读框（ORF）以及上游调控区（URR）中发现了一个核苷酸替换，并且在URR中发现了一个短缺失和添加。BPV - 8与BPV - 5在全基因组（70%）和L1 ORF（75%）中的序列高度相似以及系统发育分析是将BPV - 8归类为Epsilon乳头瘤病毒属的依据。BPV - 8的E6和E7 ORF/蛋白也显示出Epsilon乳头瘤病毒属的一些特征。然而，BPV - 8含有BPV - 5中未发现的E4 ORF。此外，BPV - 5和BPV - 8的E5蛋白的二级结构表明这些蛋白可能具有细胞转化能力。
