新疆南部地区荷斯坦奶牛中 15 型牛乳头瘤病毒的全基因组及系统进化分析。
Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow.
机构信息
College of Animal Science, Tarim University, Alar 843300, China.
Faculty of Veterinary and Agricultural Sciences, Dookie Campus, The University of Melbourne, Melbourne, VIC 3647, Australia.
出版信息
J Vet Sci. 2020 Nov;21(6):e73. doi: 10.4142/jvs.2020.21.e73.
BACKGROUND
Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes.
OBJECTIVES
The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing.
METHODS
DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software.
RESULTS
The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX₂CXCX₂C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus 1 of the genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster.
CONCLUSIONS
The results suggest that BPV type (BPV Aks-02) clustered with members of the genus as BPV 15 and were closely related to 1.
背景
牛乳头瘤是一种由牛乳头瘤病毒（BPV）引起的肿瘤性疾病，该病毒最近被分为 5 个属和至少 24 个基因型。
目的
通过采集感染牛的疣状病变组织，提取 DNA 并进行扩增子测序，确定来自中国新疆南部感染奶牛皮肤样本中的新型牛乳头瘤病毒（BPV Aks-02）的完整基因组序列。
方法
使用简并引物 FAP59 和 FAP64 进行聚合酶链反应（PCR）初步分析 DNA。使用扩增引物和测序引物通过 PCR 扩增 BPV Aks-02 的完整基因组序列。使用生物信息学软件进行序列分析和系统发育分析。
结果
BPV Aks-02 的 L1 开放阅读框（ORF）核苷酸序列与 GenBank 中 BPV-9 参考株的 L1 ORF 具有 75%的同一性。完整基因组由 7189 个碱基对（G + C 含量为 42.50%）组成，编码 5 个早期（E8、E7、E1、E2 和 E4）和 2 个晚期（L1 和 L2）基因。E7 蛋白含有一个保守的 CX₂CXCX₂C 锌结合结构域和 LxCxE 基序。在该组的不同成员中，BPV Aks-02 的完整基因组和 ORF（包括 5 个早期和 2 个晚期 ORF）序列同一性百分比更接近属 1。基于 BPV Aks-02 的 L1 ORF 的系统发育分析和序列相似性表明，该病毒与属 1 的成员聚为一簇。
结论
结果表明，BPV 型（BPV Aks-02）与属 1 的成员（如 BPV 15）聚类，并与属 1 密切相关。
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