一种新型中药配方——太空养心方对后肢去负荷大鼠骨退变的骨保护作用

Bone protection effects of a novel Chinese herbal formula, taikong yangxin prescription, in hindlimb unloaded rats against bone deterioration.

作者信息

Ko Chun-hay, Siu Wing-sum, Chan Chung-lap, Koon Chi-man, Fung Kwok-pui, Li Yong-zhi, Li Ying-hui, Leung Ping-chung

机构信息

Institute of Chinese Medicine, Chinese University of Hong Kong, Hong Kong, China.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Chinese University of Hong Kong, Hong Kong, China.

出版信息

Chin J Integr Med. 2015 Oct;21(10):759-64. doi: 10.1007/s11655-015-2310-3. Epub 2015 Nov 3.

DOI:10.1007/s11655-015-2310-3

PMID:26525547

Abstract

OBJECTIVE

To investigate the protective effects of a Chinese herbal formula, taikong yangxin prescription (TKYXP) against bone deterioration in a hindlimb unloaded (tail-suspension) rat model.

METHODS

Thirty-two male Sprague-Dawley rats were divided into 4 groups: tail-suspension group fed with 2.5 g•kg(-1)•day(-1) of TKYXP extract (high dose), tail-suspension group fed with 1.25 g•kg(-1)•day(-1) (low dose), tail-suspended group treated with water placebo (placebo control group) and non tail-suspended group. The effects of TKYXP on bone were assessed using peripheral quantitative computed tomography (pQCT), microcomputerized tomography (micro-CT) and three-point bending biomechanical test on the femur in vivo.

RESULTS

TKYXP had a significant protective effect against bone loss induced by tail-suspension on day 28, as shown in the reduction in bone mineral density (BMD) loss, preservation of bone micro-architecture and biomechanical strength. The administration ofhigh dose TKYXP could significantly reduce the total BMD loss by 4.8% and 8.0% at the femur and tibia regions, respectively, compared with the placebo control group (P<0.01) on day 28. Its bone protective effect on the femur was further substantiated by the increases of the trabecular BMD (by 6.6%), bone volume fraction (by 20.9%), trabecular number (by 9.5%) and thickness (by 11.9%) as compared with the placebo control group.

CONCLUSION

TKYXP may protect the bone under weightless influence from gradual structural deterioration in the tail-suspension model.

摘要

目的

在大鼠后肢去负荷（尾部悬吊）模型中，研究中药复方太空养心方（TKYXP）对骨质退变的保护作用。

方法

将32只雄性Sprague-Dawley大鼠分为4组：尾部悬吊高剂量组，给予2.5 g•kg⁻¹•天⁻¹的TKYXP提取物；尾部悬吊低剂量组，给予1.25 g•kg⁻¹•天⁻¹；尾部悬吊水安慰剂组（安慰剂对照组）；非尾部悬吊组。通过外周定量计算机断层扫描（pQCT）、微型计算机断层扫描（micro-CT）和三点弯曲生物力学试验，在体评估TKYXP对股骨的影响。

结果

在第28天，TKYXP对尾部悬吊诱导的骨质流失具有显著保护作用，表现为骨密度（BMD）损失减少、骨微结构和生物力学强度得以保留。与安慰剂对照组相比，高剂量TKYXP给药可使第28天股骨和胫骨区域的总BMD损失分别显著降低4.8%和8.0%（P<0.01）。与安慰剂对照组相比，股骨小梁BMD增加6.6%、骨体积分数增加20.9%、小梁数量增加9.5%、厚度增加11.9%，进一步证实了其对股骨的骨保护作用。