Zhang Guangwei, Li Chenrui, Niu Yinbo, Yu Qi, Chen Yulong, Liu Enqi
Research Institute of Atherosclerotic Disease, College of Clinical Medicine, Xi'an Medical University, No.1Xinwang Road, Xi'an 710021, China.
Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
Molecules. 2017 Jul 3;22(7):1044. doi: 10.3390/molecules22071044.
A number of medicinal herbs have demonstrated therapeutic effects for the prevention and treatment of disuse-induced osteoporosis. As a common ingredient in proprietary traditional Chinese medicines, the anti-osteoporosis effects of extract (RSE, 50 mg/kg/day) were evaluated in a hindlimb suspended rat model. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry, and the micro-architecture observed by MicroCT assay with bone biomechanical properties evaluated by a three-point bending test. To elucidate potential mechanisms, the osteogenic differentiation effect of baicalin as the most abundant ingredient in RSE was investigated in rat bone marrow derived mesenchymal stem cells (rBMSC). After drug administration for 42 days, tibia-BMD was significantly increased to 0.176 ± 0.007 and 0.183 ± 0.011 g/cm² and f-BMD was enhanced to 0.200 ± 0.017 and 0.207 ± 0.021 g/cm² for RSE and ALE treatment, respectively, whereas tibia-BMD and femur-BMD of the HLS group were 0.157 ± 0.009 and 0.176 ± 0.008 g/cm². Deterioration of bone trabecula microstructure was improved by RSE and ALE with increased morphological parameters such as bone volume fraction, trabecular thickness, and trabecular number, as well as connectivity density compared to the HLS group ( < 0.01). A three-point bending test suggested that bone mechanical strength was also enhanced by RSE and ALE treatments with increased maximum stress, young's modulus, maximum load, and stiffness compared to those of the HLS group ( < 0.05). Besides, serum TRACP levels were significantly suppressed by RSE and ALE treatments. Furthermore, in vitro studies demonstrated that baicalin significantly increased ALP activities and the formation of mineralized nodules in rBMSC. Conclusively, supplementation of RSE could significantly prevent weightlessness induced osteoporosis, which might attribute to the osteogenic differentiation enhancement effect of baicalin.
许多草药已显示出对预防和治疗废用性骨质疏松症的治疗效果。作为中成药中的常见成分，在大鼠后肢悬吊模型中评估了提取物（RSE，50毫克/千克/天）的抗骨质疏松作用。通过双能X线吸收法测量骨矿物质密度（BMD），并通过MicroCT测定观察微观结构，通过三点弯曲试验评估骨生物力学性能。为了阐明潜在机制，研究了黄芩苷作为RSE中最丰富成分在大鼠骨髓间充质干细胞（rBMSC）中的成骨分化作用。给药42天后，RSE和ALE治疗组的胫骨BMD分别显著增加至0.176±0.007和0.183±0.011克/平方厘米，股骨BMD分别提高至0.200±0.017和0.207±0.021克/平方厘米，而HLS组的胫骨BMD和股骨BMD分别为0.157±0.009和0.176±0.008克/平方厘米。与HLS组相比，RSE和ALE改善了骨小梁微观结构的恶化，增加了形态学参数，如骨体积分数、骨小梁厚度和骨小梁数量，以及连接密度（<0.01）。三点弯曲试验表明，与HLS组相比，RSE和ALE治疗还增强了骨机械强度，增加了最大应力、杨氏模量、最大负荷和刚度（<0.05）。此外，RSE和ALE治疗显著抑制了血清TRACP水平。此外，体外研究表明，黄芩苷显著增加了rBMSC中的碱性磷酸酶活性和成矿结节的形成。总之，补充RSE可以显著预防失重诱导的骨质疏松症，这可能归因于黄芩苷的成骨分化增强作用。
