Dieryckx Cindy, Gaudin Vanessa, Dupuy Jean-William, Bonneu Marc, Girard Vincent, Job Dominique
Laboratoire Mixte UMR 5240, Plateforme de Protéomique, Centre National de la Recherche Scientifique Lyon, France.
Plateforme Protéome, Centre de Génomique Fonctionnelle, Université de Bordeaux Bordeaux, France.
Front Plant Sci. 2015 Oct 16;6:859. doi: 10.3389/fpls.2015.00859. eCollection 2015.
Using Botrytis cinerea we confirmed in the present work several previous studies showing that salicylic acid, a main plant hormone, inhibits fungal growth in vitro. Such an inhibitory effect was also observed for the two salicylic acid derivatives, methylsalicylic and acetylsalicylic acid. In marked contrast, 5-sulfosalicylic acid was totally inactive. Comparative proteomics from treated vs. control mycelia showed that both the intracellular and extracellular proteomes were affected in the presence of salicylic acid or methylsalicylic acid. These data suggest several mechanisms that could potentially account for the observed fungal growth inhibition, notably pH regulation, metal homeostasis, mitochondrial respiration, ROS accumulation and cell wall remodeling. The present observations support a role played by the phytohormone SA and derivatives in directly containing the pathogen. Data are available via ProteomeXchange with identifier PXD002873.
在本研究中，我们利用灰葡萄孢证实了先前的几项研究，这些研究表明，主要植物激素水杨酸在体外可抑制真菌生长。两种水杨酸衍生物，即水杨酸甲酯和乙酰水杨酸，也观察到了这种抑制作用。相比之下，5-磺基水杨酸则完全没有活性。处理过的菌丝体与对照菌丝体的比较蛋白质组学表明，在水杨酸或水杨酸甲酯存在的情况下，细胞内和细胞外蛋白质组均受到影响。这些数据提示了几种可能解释所观察到的真菌生长抑制的机制，特别是pH调节、金属稳态、线粒体呼吸、活性氧积累和细胞壁重塑。目前的观察结果支持了植物激素水杨酸及其衍生物在直接抑制病原体方面所起的作用。数据可通过ProteomeXchange获得，标识符为PXD002873。
