Dhakal Dipesh, Pokhrel Anaya Raj, Jha Amit Kumar, Thuan Nguyen Huy, Sohng Jae Kyung
Department of Life Science and Biochemical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Center for Molecular Biology, Institute of Research and Development, Duy Tan University, Danang, Vietnam.
Curr Protoc Microbiol. 2017 Feb 6;44:10H.1.1-10H.1.13. doi: 10.1002/cpmc.21.
Saccharopolyspora spp. are aerobic, Gram-positive, non-acid-fast, and non-motile actinomycetes. Various species of the genus Saccharopolyspora have been reported with an ability to produce various bioactive compounds for pharmaceutical and agricultural uses. This unit includes general protocols for the laboratory maintenance of Saccharopolyspora species, including growth in liquid medium, growth on solid agar, long-term storage, and generation of a higher producer strain by mutagenesis. Saccharopolyspora spinosa ATCC 49460 is used as a prototype for explaining the considerations for efficient laboratory maintenance of Saccharopolyspora spp. Saccharopolyspora spinosa is a producer of spinosad, a prominent insecticide with selective activity against various insects. © 2017 by John Wiley & Sons, Inc.
糖多孢菌属是需氧、革兰氏阳性、非抗酸且无运动性的放线菌。已报道糖多孢菌属的 various species 能够产生多种用于制药和农业的生物活性化合物。本单元包括糖多孢菌属菌种实验室保藏的通用方案，包括在液体培养基中的生长、在固体琼脂上的生长、长期保存以及通过诱变产生更高产菌株。多杀菌素糖多孢菌 ATCC 49460 用作解释糖多孢菌属菌种高效实验室保藏注意事项的原型。多杀菌素糖多孢菌是多杀菌素的产生菌，多杀菌素是一种对多种昆虫具有选择性活性的著名杀虫剂。© 2017 约翰威立父子出版公司
