Calame W, Mattie H
Dept. Infectious Diseases, University Hospital, Leiden, The Netherlands.
Agents Actions. 1989 Jan;26(1-2):77-8. doi: 10.1007/BF02126564.
The effects of monocytopenia and granulocytopenia on the proliferation of Klebsiella pneumoniae and the efficacy of treatment with ceftriaxone were studied in an experimental lung infection in mice. Treatment with etoposide led to monocytopenia, and cyclophosphamide to granulocytopenia and monocytopenia. Both drugs gave a decrease in the number of alveolar macrophages during the infection and a diminished influx of granulocytes into the alveolar space. The mice treated with etoposide did not differ from controls with respect to either the proliferation of Kl. pneumoniae during the infection or the antibiotic efficacy of ceftriaxone. In cyclophosphamide-treated mice the proliferation rate of Kl. pneumoniae was higher than that in the control mice and a higher dose of ceftriaxone was needed to reduce the number of bacteria to the level in the controls. From these findings it is concluded that granulocytes play an important role in the numerical reduction of Kl. pneumoniae and that monocytes make no contribution to infection control in this model.
在小鼠实验性肺部感染中，研究了单核细胞减少症和粒细胞减少症对肺炎克雷伯菌增殖以及头孢曲松治疗效果的影响。依托泊苷治疗导致单核细胞减少，环磷酰胺导致粒细胞减少和单核细胞减少。两种药物在感染期间均使肺泡巨噬细胞数量减少，粒细胞向肺泡腔的流入减少。接受依托泊苷治疗的小鼠在感染期间肺炎克雷伯菌的增殖或头孢曲松的抗生素疗效方面与对照组无差异。在环磷酰胺治疗的小鼠中，肺炎克雷伯菌的增殖率高于对照小鼠，需要更高剂量的头孢曲松才能将细菌数量减少到对照组的水平。从这些发现可以得出结论，粒细胞在肺炎克雷伯菌数量减少中起重要作用，而在该模型中单核细胞对感染控制没有贡献。
