van't Wout J W, Linde I, Leijh P C, van Furth R
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
Inflammation. 1989 Feb;13(1):1-14. doi: 10.1007/BF00918959.
In order to develop a suitable model for studying the role of granulocytes and monocytes in resistance against pathogenic microorganisms, we investigated the effect of irradiation and cytostatic treatment (cyclophosphamide and VP-16) on the number of both peripheral blood and peritoneal leukocytes in male Swiss mice. Irradiation and cyclophosphamide treatment severely decreased the number of both granulocytes and monocytes in peripheral blood, whereas VP-16 only lowered the number of blood monocytes to a significant degree and had little effect on the number of blood granulocytes or lymphocytes. When normal mice were injected intraperitoneally with newborn calf serum (NBCS) the number of peritoneal granulocytes rose about 100-fold within 6 h. In irradiated and cyclophosphamide-treated mice, this influx of granulocytes into the peritoneal cavity was virtually eliminated, as was the concomitant increase in the number of blood granulocytes; in VP-16-treated mice, on the other hand, the number of peripheral blood and peritoneal granulocytes increased to the same degree as in normal mice. An increase in the number of peripheral blood monocytes and peritoneal macrophages occurred 24-48 h after injection of NBCS in normal mice. This increase was significantly impaired by irradiation as well as by treatment with cyclophosphamide or VP-16.
为了建立一个合适的模型来研究粒细胞和单核细胞在抵抗病原微生物中的作用，我们研究了辐射和细胞抑制治疗（环磷酰胺和依托泊苷）对雄性瑞士小鼠外周血和腹腔白细胞数量的影响。辐射和环磷酰胺治疗严重降低了外周血中粒细胞和单核细胞的数量，而依托泊苷仅显著降低了血液单核细胞的数量，对血液粒细胞或淋巴细胞的数量影响很小。当正常小鼠腹腔注射新生小牛血清（NBCS）时，腹腔粒细胞数量在6小时内增加了约100倍。在接受辐射和环磷酰胺治疗的小鼠中，粒细胞向腹腔的这种流入几乎被消除，血液粒细胞数量的相应增加也被消除；另一方面，在接受依托泊苷治疗的小鼠中，外周血和腹腔粒细胞数量增加到与正常小鼠相同的程度。在正常小鼠中，注射NBCS后24 - 48小时外周血单核细胞和腹腔巨噬细胞数量增加。这种增加受到辐射以及环磷酰胺或依托泊苷治疗的显著损害。
