Chen Wen-Ming, Su Ching-Ling, Sheu Shih-Yi
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 Apr;67(4):875-882. doi: 10.1099/ijsem.0.001693.
A novel bacterial strain AHQ-46 was isolated from a freshwater lake in Taiwan. The strain was Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20-25 °C, pH 7.0, and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AHQ-46 belonged to the genus Flavobacterium and was most closely related to Flavobacterium verecundum TTM-46 with a sequence similarity of 95.8 %. Strain AHQ-46 contained iso-C15 : 1 G, iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C17 : 0 3-OH as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 32.1 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain AHQ-46 should be classified as a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium lacunae sp. nov. is proposed. The type strain is AHQ-46 (=BCRC 80889=LMG 28710).
从台湾的一个淡水湖分离出一株新型细菌菌株AHQ - 46。该菌株革兰氏染色阴性，严格需氧，通过滑行运动，呈杆状，形成半透明黄色菌落。在20 - 25°C、pH 7.0且无氯化钠的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株AHQ - 46属于黄杆菌属，与柔弱黄杆菌TTM - 46关系最为密切，序列相似性为95.8%。菌株AHQ - 46以异C15:1 G、异C15:0、总特征3（C16:1ω6c和/或C16:1ω7c）和异C17:0 3 - OH作为主要脂肪酸。极性脂质谱由磷脂酰乙醇胺和几种未鉴定的氨基磷脂及磷脂组成。主要的多胺是高亚精胺。主要的类异戊二烯醌是MK - 6。基因组DNA的G + C含量为32.1 mol%。不同的表型特征以及系统发育推断表明，菌株AHQ - 46应被归类为黄杆菌属一个新物种的代表，为此提出新物种名称为湖泊黄杆菌（Flavobacterium lacunae sp. nov.）。模式菌株为AHQ - 46（=BCRC 80889 = LMG 28710）。
