Martin Jérôme C, Bériou Gaëlle, Josien Régis
INSERM UMR1064, ITUN, Nantes, 44000, France.
Faculté de Médecine, Université de Nantes, Nantes, 44000, France.
Methods Mol Biol. 2016;1371:197-203. doi: 10.1007/978-1-4939-3139-2_12.
Inflammatory bowel diseases (IBDs) are complex multifactorial disease thought to result from inappropriate immune responses to the gut microbiota, in genetically susceptible individuals, under the influence of environmental factors. Among the different animal models developed to help in understanding IBDs pathophysiological mechanisms as well as to achieve pharmacological preclinical studies, the dextran sulfate sodium (DSS)-induced colitis model is the most widely used because of its simplicity, cost-effectiveness, and similarity with human IBDs. This section provides with a detailed protocol that we validated in our laboratory to perform DSS-induced acute colitis in the Sprague-Dawley (SPD) rat.
炎症性肠病（IBDs）是一种复杂的多因素疾病，被认为是在环境因素的影响下，遗传易感性个体对肠道微生物群产生不适当免疫反应所致。在为帮助理解IBDs病理生理机制以及进行药理学临床前研究而开发的不同动物模型中，葡聚糖硫酸钠（DSS）诱导的结肠炎模型因其简单、成本效益高以及与人类IBDs相似而被最广泛使用。本节提供了一个详细的方案，该方案在我们实验室经过验证，用于在斯普拉格-道利（SPD）大鼠中诱导DSS引起的急性结肠炎。
