Fedulova E N, Saralov S N, Shumilova O V, Shirokova N Yu, Ilyina K N, Farafontova E A, Rubtsova Yu P, Egorikhina M N
MD, DSc, Head of the F.D. Agafonov Pediatric Department; Head of Pediatric Unit No.1, University Clinic; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
MD, PhD, Endoscopist, Consulting and Diagnostic Department, University Clinic; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
Sovrem Tekhnologii Med. 2024;16(6):34-42. doi: 10.17691/stm2024.16.6.04. Epub 2024 Dec 27.
Dissatisfactory results of treating inflammatory bowel disease require the development of new modern methods of therapy. is to create a model of Crohn's disease on large animals, in which ulcerative defects can be formed, for testing new treatment techniques and assessing their effectiveness by endoscopic and morphological methods.
The model was created and tested on 12 castrated male pigs (hybrids between the Wiesenau and the Vietnamese black potbellied pigs), aged 6 months. The animals were manipulated under general sedation in the operating room of the SPF-vivarium for large laboratory animals at Privolzhsky Research Medical University (Russia). Endoscopic techniques and a highfrequency electrosurgical apparatus were used to create the required defects. The results were assessed endoscopically and with histological and morphometrical techniques on days 7, 14, and 21.
The morphological examination of the pigs' intestinal mucous membrane has detected the signs typical of Crohn's disease, demonstrating the possibility of using pigs as a model of ulcerative defects in Crohn's disease.
This model of Crohn's disease on large animals (pigs, in particular) significantly widens the borders of using new treatment techniques at the preclinical stage and will improve therapy effectiveness in patients with this disease reducing the risk of surgical intervention.
治疗炎症性肠病的不理想结果需要开发新的现代治疗方法。目的是在大型动物上建立克罗恩病模型，在该模型中可以形成溃疡性缺损，用于测试新的治疗技术并通过内镜和形态学方法评估其有效性。
该模型在12头6月龄去势雄性猪（威森瑙猪和越南黑腹猪的杂交种）上建立并进行测试。动物在俄罗斯下诺夫哥罗德研究医科大学大型实验动物无特定病原体动物饲养室的手术室中，在全身麻醉下进行操作。使用内镜技术和高频电外科设备制造所需的缺损。在第7天、14天和21天通过内镜以及组织学和形态计量学技术评估结果。
对猪肠黏膜的形态学检查发现了克罗恩病的典型体征，证明了将猪用作克罗恩病溃疡性缺损模型的可能性。
这种大型动物（特别是猪）的克罗恩病模型显著拓宽了临床前阶段使用新治疗技术的范围，并将提高该疾病患者的治疗效果，降低手术干预风险。
