Shangguan J B, Li Z B, Yuan Y, Huang Y S
Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Fisheries College, Jimei University, Xiamen, China.
Genet Mol Res. 2015 Oct 28;14(4):13582-7. doi: 10.4238/2015.October.28.18.
Tropical commercial sea cucumber Stichopus horrens is extensively distributed throughout the tropical Indo-Pacific region, and wild stocks have been severely depleted over the past decade. In this study, we used the microsatellite enrichment library of S. horrens to identify and characterize 13 microsatellite loci, including 11 polymorphic loci and 2 monomorphic loci. Among the 11 polymorphic loci, the number of alleles was 3-8. The observed and expected heterozygosity varied from 0.1364 to 0.8966 and from 0.1653 to 0.7551, respectively. Additionally, all 11 polymorphic loci showed moderate and high polymorphism with the polymorphism information content (0.271-0.7311). A total of 9 polymorphic loci were in Hardy-Weinberg equilibrium, except for 2 loci (adjusted P = 0.004545). Linkage disequilibrium was not detected in any pairs of polymorphic loci. The present study will be useful for studying genetic structure, population conservation, and breeding of wild S. horrens; moreover, our results contribute to the phylogeny and evolutionary research of Holothuroidea.
热带商业海参棘辐肛参广泛分布于整个热带印度洋 - 太平洋地区，在过去十年中，野生种群已严重枯竭。在本研究中，我们利用棘辐肛参的微卫星富集文库来鉴定和表征13个微卫星位点，其中包括11个多态性位点和2个单态性位点。在这11个多态性位点中，等位基因数量为3 - 8个。观察到的杂合度和预期杂合度分别在0.1364至0.8966和0.1653至0.7551之间变化。此外，所有11个多态性位点均表现出中度和高度多态性，多态性信息含量为0.271 - 0.7311。除2个位点外（校正P = 0.004545），共有9个多态性位点处于哈迪 - 温伯格平衡。在任何多态性位点对中均未检测到连锁不平衡。本研究将有助于研究野生棘辐肛参的遗传结构、种群保护和育种；此外，我们的结果有助于海参纲的系统发育和进化研究。
