Richards Sarah-Jane, Biggs Caroline I, Gibson Matthew I
Department of Chemistry, The University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK.
Methods Mol Biol. 2016;1367:169-79. doi: 10.1007/978-1-4939-3130-9_14.
Glycosylated noble metal nanoparticles are a useful tool for probing biological binding events due to their aggregation-induced color changes, particularly for lectins that have multiple binding sites. To overcome the challenges of colloidal instability, which leads to false-positive results, it is essential to add polymeric coatings to these particles. Here we describe a versatile, and reliable, approach to enable coating of gold nanoparticles using well-defined polymers, with carbohydrate end groups. This produces multivalent nanoparticles that are both colloidally stable, but still retain their rapid colorimetric responses to lectin binding.
糖基化贵金属纳米颗粒因其聚集诱导的颜色变化，是探测生物结合事件的有用工具，尤其适用于具有多个结合位点的凝集素。为了克服导致假阳性结果的胶体不稳定性挑战，给这些颗粒添加聚合物涂层至关重要。在此，我们描述了一种通用且可靠的方法，可使用具有碳水化合物端基的明确聚合物对金纳米颗粒进行涂层。这产生了多价纳米颗粒，它们既具有胶体稳定性，又仍保留对凝集素结合的快速比色响应。
