van Lohuizen M, Breuer M, Berns A
Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam.
EMBO J. 1989 Jan;8(1):133-6. doi: 10.1002/j.1460-2075.1989.tb03357.x.
We report a new common proviral insertion site in murine leukemia virus-induced T cell lymphomas to be N-myc. Proviral activation of N-myc was found in 35% of independently induced primary tumors. The vast majority of the proviral insertions occur within a small segment of the 3'-untranslated region of the N-myc gene, directly downstream of the protein-encoding domain. This results in an increased level of expression of a truncated N-myc mRNA. Together with the previously shown c-myc activation we now find involvement of myc genes in greater than 75% of the primary T cell lymphomas induced by Moloney murine leukemia virus in C57BL10 and BALB/c mice, and show for the first time that N-myc can be over-expressed by a mechanism other than gene amplification.
我们报告在鼠白血病病毒诱导的T细胞淋巴瘤中一个新的常见原病毒插入位点为N-myc。在35%的独立诱导的原发性肿瘤中发现了N-myc的原病毒激活。绝大多数原病毒插入发生在N-myc基因3'-非翻译区的一小段内，直接位于蛋白质编码结构域的下游。这导致截短的N-myc mRNA表达水平增加。连同之前显示的c-myc激活，我们现在发现在C57BL10和BALB/c小鼠中，超过75%的由莫洛尼鼠白血病病毒诱导的原发性T细胞淋巴瘤涉及myc基因，并且首次表明N-myc可以通过基因扩增以外的机制过度表达。
