Selten G, Cuypers H T, Zijlstra M, Melief C, Berns A
EMBO J. 1984 Dec 20;3(13):3215-22. doi: 10.1002/j.1460-2075.1984.tb02281.x.
In approximately 45% of the murine leukemia virus (MuLV) induced early developing T cell lymphomas in mice, integration of proviruses occurs near c-myc. From the 33 lymphomas with proviral integrations in the c-myc domain, 29 insertions were localized upstream of the first exon in a region spanning less than 2 kbp, and four integrations occurred within the first exon. In 90% of the lymphomas the transcriptional orientation of the proviruses was opposite to the transcriptional direction of c-myc. In 20% of the early T cell lymphomas, proviral integrations were detected both near c-myc and the pim-1 gene. They comprise both lymphomas in which integration near c-myc and pim-1 occurred in separate tumor cell populations, as well as tumors in which proviral integration near c-myc and pim-1 occurred in the same cell clone. Proviral integration in the c-myc domain is associated with increased myc mRNA levels (up to 30-fold). The size and nature of the c-myc mRNA precursors and processed transcripts depend on the position and orientation of the integrated proviruses.
在小鼠中，约45%由鼠白血病病毒（MuLV）诱导的早期发育性T细胞淋巴瘤中，前病毒整合发生在c-myc附近。在c-myc结构域有前病毒整合的33个淋巴瘤中，29个插入位于第一个外显子上游小于2 kbp的区域，4个整合发生在第一个外显子内。在90%的淋巴瘤中，前病毒的转录方向与c-myc的转录方向相反。在20%的早期T细胞淋巴瘤中，在c-myc和pim-1基因附近均检测到前病毒整合。它们既包括c-myc和pim-1附近的整合发生在不同肿瘤细胞群体中的淋巴瘤，也包括c-myc和pim-1附近的前病毒整合发生在同一细胞克隆中的肿瘤。c-myc结构域中的前病毒整合与myc mRNA水平升高（高达30倍）相关。c-myc mRNA前体和加工转录本的大小和性质取决于整合前病毒的位置和方向。
