Pulford K A, Rigney E M, Micklem K J, Jones M, Stross W P, Gatter K C, Mason D Y
Nuffield Department of Pathology, John Radcliffe Hospital, Oxford.
J Clin Pathol. 1989 Apr;42(4):414-21. doi: 10.1136/jcp.42.4.414.
A new monoclonal antibody, KP1, raised against a lysosomal fraction of human lung macrophages, recognises a fixation-resistant epitope in a wide variety of tissue macrophages (such as Kupffer cells germinal centre, splenic, and lamina propria macrophages), and in granulocyte precursors. Its broad reactivity with cells of the mononuclear phagocytic lineage was established by testing on routinely processed samples of normal and reactive lymphoid tissues. Interdigitating reticulum cells were unstained or showed limited cytoplasmic staining while Langerhans' cells and follicular dendritic reticulum cells were unreactive. KP1 recognises a molecule of about 110 kilodaltons in macrophage-rich human tissue when tested by either immunoprecipitation or Western blotting (although the latter procedure also shows two additional components with molecular weights of 70 and 40 kilodaltons). KP1 should be of considerable value for studying disorders of the monocyte/macrophage system, including both reactive and neoplastic states (such as true histiocytic proliferations).
一种新的单克隆抗体KP1，是针对人肺巨噬细胞的溶酶体部分产生的，它能识别多种组织巨噬细胞（如库普弗细胞、生发中心、脾和固有层巨噬细胞）以及粒细胞前体中一种抗固定表位。通过对正常和反应性淋巴组织的常规处理样本进行检测，确定了它与单核吞噬细胞系细胞的广泛反应性。交错突网状细胞不着色或仅显示有限的细胞质染色，而朗格汉斯细胞和滤泡树突状网状细胞无反应。当通过免疫沉淀或蛋白质印迹法检测时，KP1在富含巨噬细胞的人体组织中识别出一种约110千道尔顿的分子（尽管后者方法还显示出另外两种分子量分别为70和40千道尔顿的成分）。KP1对于研究单核细胞/巨噬细胞系统的疾病，包括反应性和肿瘤性状态（如真性组织细胞增生症）具有重要价值。
