Gopinath Kulasekaran, Sudhandiran Ganapasam
Department of Biochemistry, Cell Biology Laboratory, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
Can J Physiol Pharmacol. 2016 Jan;94(1):65-71. doi: 10.1139/cjpp-2015-0035. Epub 2015 Jun 23.
Naringin (4',5,7-trihydroxy-flavonone-7-rhamnoglucoside), a flavonone present in grapefruit, has recently been reported to protect against neurodegeration, induced with 3-nitropropionic acid (3-NP), through its antioxidant, anti-inflammatory, and antiapoptotic properties. This study used a rat model of 3-NP-induced neurodegeneration to investigate the neuroprotective effects of naringin exerted by modulating the expression of matrix metalloproteinases and glial fibrillary acidic protein. Neurodegeneration was induced with 3-NP (10 mg/kg body mass, by intraperitoneal injection) once a day for 2 weeks, and induced rats were treated with naringin (80 mg/kg body mass, by oral gavage, once a day for 2 weeks). Naringin ameliorated the motor abnormalities caused by 3-NP, and reduced blood-brain barrier dysfunction by decreasing the expression of matrix metalloproteinases 2 and 9, along with increasing the expression of the tissue inhibitors of metalloproteinases 1 and 2 in 3-NP-induced rats. Further, naringin reduced 3-NP-induced neuroinflammation by decreasing the expression of nuclear factor-kappa B and glial fibrillary acidic protein. Thus, naringin exerts protective effects against 3-NP-induced neurodegeneration by ameliorating the expressions of matrix metalloproteinases and glial fibrillary acidic protein.
柚皮苷（4',5,7-三羟基黄酮-7-鼠李糖葡萄糖苷）是一种存在于葡萄柚中的黄酮类化合物，最近有报道称，它具有抗氧化、抗炎和抗凋亡特性，可预防由3-硝基丙酸（3-NP）诱导的神经退行性变。本研究使用3-NP诱导的神经退行性变大鼠模型，探讨柚皮苷通过调节基质金属蛋白酶和胶质纤维酸性蛋白的表达所发挥的神经保护作用。通过腹腔注射3-NP（10 mg/kg体重）诱导神经退行性变，每天1次，持续2周，对诱导的大鼠用柚皮苷（80 mg/kg体重，经口灌胃，每天1次，持续2周）进行治疗。柚皮苷改善了3-NP引起的运动异常，并通过降低基质金属蛋白酶2和9的表达以及增加金属蛋白酶组织抑制剂1和2的表达，减轻了血脑屏障功能障碍。此外，柚皮苷通过降低核因子-κB和胶质纤维酸性蛋白的表达，减轻了3-NP诱导的神经炎症。因此，柚皮苷通过改善基质金属蛋白酶和胶质纤维酸性蛋白的表达，对3-NP诱导的神经退行性变发挥保护作用。
