Buoro S, Mecca T, Azzarà G, Seghezzi M, Candiago E, Gianatti A, Crippa A, La Gioia A
Chimical Chemistry Laboratory, Hospital Papa Giovanni XXIII, Bergamo, Italy.
Pathology Laboratory, Hospital Papa Giovanni XXIII, Bergamo, Italy.
Int J Lab Hematol. 2016 Feb;38(1):90-101. doi: 10.1111/ijlh.12446. Epub 2015 Nov 7.
An accurate and rapid analysis of cells in body fluids (BFs) is important for diagnosis and follow-up in many pathological conditions. We evaluated the analytical performance of the module BF Mindray BC-6800 (BC-6800-BF) for cytometric analysis of ascitic and pleural fluids.
A total of 99 ascitic and 45 pleural samples were collected and assessed with BC-6800-BF and optical microscopy. This study also includes the evaluation of limit blank (LoB), limit detection (LoD), limit quantitation, (LoQ), carryover, linearity, and diagnostic concordance between the two methods.
For TC-BF, LoB was 1 × 10(6) cells/L, LoD was 3 × 10(6) cells/L, and LoQ was 4 × 10(6) cells/L. Linearity was excellent (r(2) = 0.99) and carryover was negligible. TC-BF performed with the two methods showed Pearson's correlation of 0.99 (P < 0.0001), Passing-Bablok regression y = 1.04x - 1.17, and bias 33.7 cells. In ascitic fluids, polymorphonuclear cells (PMN) showed an area under curve (AUC) of 0.98 (P < 0.0001). In pleural fluids, mononuclear cells (MN) and PMN % displayed an AUC of 0.79 (P < 0.0001) and 0.93 (P < 0.0001), respectively.
BC-6800-BF in ascitic and pleural fluids offers rapid and accurate cell and differential counts in clinically relevant concentration ranges. The use of BC-6800-BF may allow to replace routine optical counting, except for samples displaying abnormal cell counts or abnormal DIFF scattergram.
准确快速地分析体液中的细胞对于多种病理状况的诊断和随访至关重要。我们评估了迈瑞BC-6800模块（BC-6800-BF）用于腹水和胸水细胞计数分析的性能。
共收集了99份腹水样本和45份胸水样本，并用BC-6800-BF和光学显微镜进行评估。本研究还包括评估检测限（LoB）、检测下限（LoD）、定量下限（LoQ）、携带污染、线性以及两种方法之间的诊断一致性。
对于总细胞数（TC-BF），LoB为1×10⁶个细胞/升，LoD为3×10⁶个细胞/升，LoQ为4×10⁶个细胞/升。线性良好（r² = 0.99），携带污染可忽略不计。两种方法检测TC-BF的结果显示Pearson相关性为0.99（P < 0.0001），Passing-Bablok回归方程为y = 1.04x - 1.17，偏差为33.7个细胞。在腹水中，多形核细胞（PMN）的曲线下面积（AUC）为0.98（P < 0.0001）。在胸水中，单核细胞（MN）和PMN%的AUC分别为0.79（P < 0.0001）和0.93（P < 0.0001）。
BC-6800-BF对腹水和胸水进行细胞计数及分类计数，在临床相关浓度范围内快速准确。除细胞计数异常或DIFF散点图异常的样本外，使用BC-6800-BF可能无需进行常规光学计数。
