Alcaide Martín María José, Altimira Queral Laura, Sahuquillo Frías Laura, Valiña Amado Laura, Merino Anna, García de Guadiana-Romualdo Luis
Committee of Laboratory Tests, Spanish Society of Laboratory Medicine (SEQC-ML), Barcelona, Spain.
Hematologic Biology Committee, Spanish Society of Laboratory Medicine (SEQC-ML), Barcelona, Spain.
Adv Lab Med. 2021 Mar 15;2(2):149-177. doi: 10.1515/almed-2021-0011. eCollection 2021 May.
Body fluid cell counting provides valuable information for the diagnosis and treatment of a variety of conditions. Chamber cell count and cellularity analysis by optical microscopy are considered the gold-standard method for cell counting. However, this method has a long turnaround time and limited reproducibility, and requires highly-trained personnel. In the recent decades, specific modes have been developed for the analysis of body fluids. These modes, which perform automated cell counting, are incorporated into hemocytometers and urine analyzers. These innovations have been rapidly incorporated into routine laboratory practice. At present, a variety of analyzers are available that enable automated cell counting for body fluids. Nevertheless, these analyzers have some limitations and can only be operated by highly-qualified laboratory professionals. In this review, we provide an overview of the most relevant automated cell counters currently available for body fluids, the interpretation of the parameters measured by these analyzers, their main analytical features, and the role of optical microscopy as automated cell counters gain ground.
体液细胞计数为多种病症的诊断和治疗提供了有价值的信息。通过光学显微镜进行的计数板细胞计数和细胞浓度分析被认为是细胞计数的金标准方法。然而，该方法周转时间长、重复性有限，且需要训练有素的人员。近几十年来，已开发出用于体液分析的特定模式。这些进行自动细胞计数的模式被整合到血细胞计数板和尿液分析仪中。这些创新已迅速融入常规实验室实践。目前，有多种分析仪可用于体液的自动细胞计数。然而，这些分析仪存在一些局限性，且只能由高素质的实验室专业人员操作。在本综述中，我们概述了目前最相关的可用于体液的自动细胞计数器、这些分析仪所测参数的解读、它们的主要分析特征，以及随着自动细胞计数器的普及光学显微镜的作用。
