保加利亚乳杆菌L3来源的β-半乳糖苷酶对甾体皂苷进行半乳糖基化修饰
Galactosylation of steroidal saponins by β-galactosidase from Lactobacillus bulgaricus L3.
作者信息
Zhang Jie, Lu Lili, Lu Li, Zhao Yang, Kang Liping, Pang Xu, Liu Jingyuan, Jiang Tengchuan, Xiao Min, Ma Baiping
机构信息
Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.
Ovation Health Science and Technology Co. Ltd., ENN Group, Langfang, 065001, China.
出版信息
Glycoconj J. 2016 Feb;33(1):53-62. doi: 10.1007/s10719-015-9632-4. Epub 2015 Nov 7.
The galactosylation of furostanosides and spirostanosides were investigated by using β-galactosidase from Lactobacillus bulgaricus L3 as a catalyst and lactose as a sugar donor. Four novel galactosylated furostanoside products (compounds 1-4) from compound F, compound G, compound I, and compound H were obtained and their structures were identified by HR-ESI-MS, 1D and 2D NMR spectra. The result showed that galactosyl moiety was found to be added to the 6-OH of the 26-O-glucosyl in these four furostanoside substrates.
以保加利亚乳杆菌L3的β-半乳糖苷酶为催化剂、乳糖为糖供体，对呋甾皂苷和螺甾皂苷的半乳糖基化反应进行了研究。从化合物F、化合物G、化合物I和化合物H中得到了4种新型半乳糖基化呋甾皂苷产物（化合物1 - 4），并通过高分辨电喷雾电离质谱（HR-ESI-MS）、一维和二维核磁共振谱对其结构进行了鉴定。结果表明，在这4种呋甾皂苷底物中，半乳糖基部分被添加到26-O-葡萄糖基的6-OH上。
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