Tadokoro K, Ueda M, Ohshima T, Fujita K, Rikimaru K, Takahashi N, Enomoto S, Tsuchida N
Department of Oral Microbiology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Oncogene. 1989 Apr;4(4):499-505.
By NIH3T3 transfection assay in conjunction with in vitro transient neomycin selection, activated c-H-ras-1 oncogenes were detected in two squamous cell carcinoma cell lines, ZA and HOC-313, newly established from human oral cancer patients. ZA had a point mutational activation at the 13th codon, this activation of c-H-ras-1 being novel in human cancer cells, while HOC-313 appeared to have an activation at the 12th codon. In ZA, 16- to 32-fold amplification of the EGF receptor gene, c-erbB-1 and a few-fold amplification of c-myc were detected. The significance of these findings is discussed in relation to multistep carcinogenesis in human cells.
通过NIH3T3转染试验并结合体外瞬时新霉素筛选，在从口腔癌患者新建立的两种鳞状细胞癌细胞系ZA和HOC-313中检测到了激活的c-H-ras-1癌基因。ZA在第13密码子处有一个点突变激活，这种c-H-ras-1的激活在人类癌细胞中是新发现的，而HOC-313似乎在第12密码子处有激活。在ZA中，检测到表皮生长因子受体基因c-erbB-1有16至32倍的扩增以及c-myc有几倍的扩增。结合人类细胞中的多步骤致癌作用对这些发现的意义进行了讨论。
