Zhang Xiaoming, Dai Luru, Wang Anhe, Wölk Christian, Dobner Bodo, Brezesinski Gerald, Tang Yunqing, Wang Xianyou, Li Junbai
National Center for Nanoscience and Technology, 100190 Beijing, China.
National Lab for Molecular Sciences, CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, 100190 Beijing, China.
Sci Rep. 2015 Nov 9;5:16559. doi: 10.1038/srep16559.
Highly dynamic tubular structures in cells are responsible for exchanges between organelles. Compared with bacterial invasion, the most affordable and least toxic lipids were found in this study to be gentle and safe exogenous stimuli for the triggering of membrane tubules. A specific lipid system was internalized by NIH3T3 cells. Following cellular uptake, the constructed liposomes traveled towards the nucleus in aggregations and were gradually distributed into moving vesicles and tubules in the cytosol. The triggered tubules proceeded, retreated or fluctuated along the cytoskeleton and were highly dynamic, moving quickly (up to several microns per second), and breaking and fusing frequently. These elongated tubules could also fuse with one another, giving rise to polygonal membrane networks. These lipid systems, with the novel property of accelerating intracellular transport, provide a new paradigm for investigating cellular dynamics.
细胞中高度动态的管状结构负责细胞器之间的物质交换。与细菌入侵相比，本研究发现最经济实惠且毒性最小的脂质是触发膜小管的温和安全的外源性刺激物。一种特定的脂质系统被NIH3T3细胞内化。细胞摄取后，构建的脂质体聚集向细胞核移动，并逐渐分布到细胞质中移动的囊泡和小管中。触发的小管沿着细胞骨架前进、后退或波动，具有高度动态性，移动迅速（每秒可达几微米），且频繁断裂和融合。这些细长的小管也可相互融合，形成多边形膜网络。这些具有加速细胞内运输新特性的脂质系统为研究细胞动力学提供了新的范例。
