Logothetopoulos J, Valiquette N, Madura E, Cvet D
Diabetes. 1984 Jan;33(1):33-6. doi: 10.2337/diab.33.1.33.
A specially developed clamping procedure permitted the easy, complication-free removal of splenic pancreas from rats. Using this biopsy procedure pancreatic tissue was removed from 50- to 90-day-old BB rats to study in a retrospective experimental design the time at which insulitis appears in BB rats, which develop acute, overt diabetes before the age of 120 days. Islets in biopsies taken 18-53 days before the onset of diabetes showed normal structure and were free from any mononuclear infiltrations. Biopsies removed between 2 and 9 days before onset of diabetes in contrast showed widespread insulitis. In five rats in which the biopsy preceded the manifestation of diabetes by 11-16 days, only a small number of pancreatic islets showed small focal mononuclear cell infiltrations. Most of the islets in these five rats had a normal histologic appearance. Thus the lesions within the islets develop rapidly starting about 2-3 wk before overt diabetes. As revealed by autoradiography, pancreatic beta-cells still surviving at the time of onset of diabetes show a modest increase in replicative activity. Replicative activity of mononuclear inflammatory cells also was observed, suggesting that their accumulation within the islet tissue may result in part from local replication.
一种专门研发的钳夹程序使得能够轻松、无并发症地从大鼠身上摘除脾胰腺。采用这种活检程序，从50至90日龄的BB大鼠身上取出胰腺组织，以回顾性实验设计研究120日龄前会发展为急性显性糖尿病的BB大鼠中胰岛炎出现的时间。在糖尿病发病前18至53天所取活检样本中的胰岛结构正常，无任何单核细胞浸润。相比之下，在糖尿病发病前2至9天取出的活检样本显示广泛的胰岛炎。在5只活检样本比糖尿病表现提前11至16天的大鼠中，只有少数胰岛显示有小灶性单核细胞浸润。这5只大鼠中的大多数胰岛组织学外观正常。因此，胰岛内的病变在显性糖尿病出现前约2至3周开始迅速发展。放射自显影显示，糖尿病发病时仍存活的胰腺β细胞复制活性适度增加。还观察到单核炎性细胞的复制活性，这表明它们在胰岛组织内的积聚可能部分源于局部复制。
