Robertson H D, Dickson E, Dunn J J
Proc Natl Acad Sci U S A. 1977 Mar;74(3):822-6. doi: 10.1073/pnas.74.3.822.
Transcription of that portion of the bacteriophage T7 genome encoding early functions yields RNA molecules about 7500 nucleotides long representing this entire early region. These long transcripts can be cleaved in vitro by highly purified Escherichia coli ribonuclease III (endoribonuclease III; EC 3.1.4.24), yielding five messenger RNAs identical to those produced in vivo. During this reaction, a small RNA fragment called F5 RNA is released, which is specified by the region of the T7 genome between genes 1.1 and 1.3. The following sequence of 32P-labeled F5 RNA has been determined using standard RNA sequencing techniques: pU-A-A-G-G-U-C-G-C-U-C-U-C-U-A-G-G-A-G-U-G-G-C-C-U-U-A-G-Uoh. The relative contributions of sequence and structure to ribonuclease III processing signals are considered in light of these findings.
噬菌体T7基因组中编码早期功能的那部分转录产生约7500个核苷酸长的RNA分子，代表了整个早期区域。这些长转录本可在体外被高度纯化的大肠杆菌核糖核酸酶III（核糖核酸内切酶III；EC 3.1.4.24）切割，产生五种与体内产生的信使RNA相同的信使RNA。在这个反应过程中，会释放出一个名为F5 RNA的小RNA片段，它由T7基因组中基因1.1和1.3之间的区域所指定。使用标准的RNA测序技术已确定了以下32P标记的F5 RNA序列：pU-A-A-G-G-U-C-G-C-U-C-U-C-U-A-G-G-A-G-U-G-G-C-C-U-U-A-G-Uoh。根据这些发现，我们考虑了序列和结构对核糖核酸酶III加工信号的相对贡献。
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