Plamann M D, Stauffer L T, Urbanowski M L, Stauffer G V
Nucleic Acids Res. 1983 Apr 11;11(7):2065-75. doi: 10.1093/nar/11.7.2065.
The nucleotide sequence of the Escherichia coli glyA gene has been determined. The amino acid sequence predicted from the DNA sequence consists of 417 residues. After the coding region there is a 185 nucleotide sequence preceding the proposed transcription termination region for the glyA gene. This region is preceded by a G-C rich sequence that could form a stable stem-loop structure once transcribed, followed by an A-T rich sequence within which transcription appears to terminate. There is a long region of dyad symmetry and numerous smaller symmetrical regions between the site of translation termination and the proposed transcription termination region. These stem-loop structures show remarkable homology with intercistronic elements of other prokaryotic operons and may play a role in the regulation of glyA gene expression.
已确定大肠杆菌glyA基因的核苷酸序列。根据DNA序列预测的氨基酸序列由417个残基组成。在编码区之后，glyA基因的假定转录终止区之前有一个185个核苷酸的序列。该区域之前是一个富含G-C的序列，一旦转录，该序列可形成稳定的茎环结构，随后是一个富含A-T的序列，转录似乎在该序列内终止。在翻译终止位点和假定的转录终止区之间有一个长的二元对称区域和许多较小的对称区域。这些茎环结构与其他原核操纵子的顺反子间元件具有显著的同源性，可能在glyA基因表达的调控中发挥作用。
