噬菌体MS2 RNA的3'末端核苷酸序列（n = 361）

3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.

作者信息

Vandenberghe A, Min Jou W, Fiers W

出版信息

Proc Natl Acad Sci U S A. 1975 Jul;72(7):2559-62. doi: 10.1073/pnas.72.7.2559.

DOI:10.1073/pnas.72.7.2559

PMID:809766

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432808/

Abstract

32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.

摘要

用核糖核酸酶T1（鸟嘌呤核糖核酸酶；核糖核酸3'-鸟嘌呤寡核苷酸水解酶；EC 3.1.4.8）或ε-羧甲基赖氨酸-41胰核糖核酸酶A（核糖核酸3'-嘧啶寡核苷酸水解酶；EC 3.1.4.22）对32P标记的MS2 RNA进行部分消化。获得了一系列重叠片段，这些片段使得能够重建一个361个核苷酸长的3'末端序列。可以推断出一个独特的阅读框，这表明复制酶基因以U-A-G终止信号结束，随后是一个174个核苷酸长的非翻译区段。