530纳米单色光对穆勒细胞中碱性成纤维细胞生长因子和转化生长因子-β1表达的影响
Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells.
作者信息
Fu Xin-Yi, Zhang Xiao-Feng, Xia Wei, Zhong Lei, Wang Ying-Ming, Sun Zheng-Tai, Xia Jing
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China.
出版信息
Int J Ophthalmol. 2015 Oct 18;8(5):904-9. doi: 10.3980/j.issn.2222-3959.2015.05.09. eCollection 2015.
AIM
To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.
METHODS
Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.
RESULTS
The bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).
CONCLUSION
The expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.
目的
将大鼠视网膜Müller细胞暴露于530nm单色光下，研究不同光照时间对碱性成纤维细胞生长因子（bFGF）和转化生长因子-β1（TGF-β1）表达的影响。
方法
将体外培养于530nm单色光下的三组大鼠视网膜Müller细胞分为6、12和24小时实验组，而在黑暗条件下孵育的细胞作为对照组。分析Müller细胞的bFGF和TGF-β1 mRNA表达、蛋白质水平及荧光强度。
结果
与对照组相比，所有三个实验组的Müller细胞中bFGF mRNA表达和蛋白质水平均显著上调（P<0.05），而TGF-β1的表达下调（P<0.05）。此外，bFGF表达呈正相关，而TGF-β1表达与光照时间呈负相关。两种细胞因子在24小时组中变化最大。bFGF和TGF-β1荧光强度在24小时组中变化最高，且实验组间观察到显著差异（P<0.05）。
结论
在光照强度为250 lx的530nm单色光照射下，Müller细胞中bFGF和TGF-β1的表达呈时间依赖性变化。Müller细胞可能通过增加bFGF表达或降低TGF-β1表达在近视发展中发挥作用。视网膜Müller细胞中细胞因子表达的变化可能影响单色光诱导的近视。
Zotero 插件