Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
Graduate School of Agricultural Science, Tohoku University , Sendai 981-8555, Japan.
Org Lett. 2015 Nov 20;17(22):5658-61. doi: 10.1021/acs.orglett.5b02934. Epub 2015 Nov 11.
To elucidate the general biosynthetic pathway of fungal dimeric anhydrides, a gene cluster for the biosynthesis of the antihy-percholesterolemic agent phomoidride was identified by heterologous expression of candidate genes encoding the highly reducing polyketide synthase, alkylcitrate synthase (ACS), and alkylcitrate dehydratase (ACDH). An in vitro analysis of ACS and ACDH revealed that they give rise to anhydride monomers. Based on the established monomer biosynthesis, we propose a general biogenesis of dimeric anhydrides involving a single donor unit and four acceptor units.
为了阐明真菌二聚酐的一般生物合成途径，通过异源表达候选基因编码的高度还原聚酮合酶、烷基柠檬酸合酶（ACS）和烷基柠檬酸脱水酶（ACDH），鉴定了用于合成抗高胆固醇血症剂 phomoidride 的基因簇。ACS 和 ACDH 的体外分析表明它们产生酐单体。基于已建立的单体生物合成，我们提出了涉及单个供体单元和四个受体单元的二聚酐的一般生物发生。
