Bahl C P, Wu R, Stawinsky J, Narang S A
Proc Natl Acad Sci U S A. 1977 Mar;74(3):966-70. doi: 10.1073/pnas.74.3.966.
A number of specific duplex DNA sequences which correspond to varying lengths of the lactose operator region have been synthesized by a combination of chemical and enzymatic methods. Repressor binding studies on these synthetic duplex operator molecules show that all the nucleotides essential for full lactose operator-repressor interactions are included in a 17-nucleotide-long duplex DNA that constitutes the minimal recognition sequence for this DNA-protein interaction.
通过化学和酶促方法相结合,已合成了许多与乳糖操纵子区域不同长度相对应的特定双链DNA序列。对这些合成双链操纵子分子的阻遏物结合研究表明,乳糖操纵子-阻遏物充分相互作用所必需的所有核苷酸都包含在一个17个核苷酸长的双链DNA中,该双链DNA构成了这种DNA-蛋白质相互作用的最小识别序列。