Marsico Alyssa L M, Elci Gokhan S, Moyano Daniel F, Yesilbag Tonga Gulen, Duncan Bradley, Landis Ryan F, Rotello Vincent M, Vachet Richard W
Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
Anal Chem. 2015 Dec 15;87(24):12145-50. doi: 10.1021/acs.analchem.5b02985. Epub 2015 Nov 24.
Laser desorption/ionization mass spectrometry (LDI-MS) has been used to detect gold nanoparticles (AuNPs) in biological samples, such as cells and tissues, by ionizing their attached monolayer ligands. Many NP-attached ligands, however, are difficult to ionize by LDI, making it impossible to track these NPs in biological samples. In this work, we demonstrate that concentrations of matrix-assisted LDI (MALDI) matrices an order of magnitude below the values typically used in MALDI can facilitate the selective detection of AuNPs with these ligands, even in samples as complex as cell lysate. This enhanced sensitivity arises from a synergistic relationship between the gold core and the matrix that helps to selectively ionize ligands attached to the AuNPs.
激光解吸/电离质谱法（LDI-MS）已被用于通过电离附着在生物样品（如细胞和组织）上的单层配体来检测金纳米颗粒（AuNPs）。然而，许多附着纳米颗粒的配体很难通过LDI进行电离，这使得在生物样品中追踪这些纳米颗粒变得不可能。在这项工作中，我们证明，基质辅助激光解吸/电离（MALDI）基质的浓度比MALDI中通常使用的值低一个数量级，即使在像细胞裂解液这样复杂的样品中，也能促进对带有这些配体的金纳米颗粒的选择性检测。这种增强的灵敏度源于金核与基质之间的协同关系，有助于选择性地电离附着在金纳米颗粒上的配体。
