Pajic-Lijakovic Ivana
Faculty of Technology and Metallurgy, Belgrade University, Karnegijeva 4, Belgrade, Serbia.
J Bioenerg Biomembr. 2015 Dec;47(6):507-18. doi: 10.1007/s10863-015-9633-9. Epub 2015 Nov 11.
An attempt was made to discuss and connect various modeling approaches on various time and space scales which have been proposed in the literature in order to shed further light on the erythrocyte membrane rearrangement caused by the cortex-lipid bilayer coupling under thermal fluctuations. Roles of the main membrane constituents: (1) the actin-spectrin cortex, (2) the lipid bilayer, and (3) the trans membrane protein band 3 and their course-consequence relations were considered in the context of the cortex non linear stiffening and corresponding anomalous nature of energy dissipation. The fluctuations induce alternating expansion and compression of the membrane parts in order to ensure surface and volume conservation. The membrane structural changes were considered within two time regimes. The results indicate that the cortex non linear stiffening and corresponding anomalous nature of energy dissipation are related to the spectrin flexibility distribution and the rate of its changes. The spectrin flexibility varies from purely flexible to semi flexible. It is influenced by: (1) the number of band 3 molecules attached to single spectrin filaments, and (2) phosphorylation of the actin-junctions. The rate of spectrin flexibility changes depends on the band 3 molecules rearrangement.
本文尝试讨论并联系文献中提出的各种时间和空间尺度上的建模方法，以便进一步阐明热涨落下皮层 - 脂质双层耦合引起的红细胞膜重排。在皮层非线性硬化及相应的能量耗散异常性质的背景下，考虑了主要膜成分的作用：（1）肌动蛋白 - 血影蛋白皮层，（2）脂质双层，以及（3）跨膜蛋白带3及其因果关系。涨落会引起膜部分的交替膨胀和压缩，以确保表面积和体积守恒。膜结构变化在两种时间状态下进行了考量。结果表明，皮层非线性硬化及相应的能量耗散异常性质与血影蛋白的柔韧性分布及其变化速率有关。血影蛋白的柔韧性从完全柔韧到半柔韧不等。它受到以下因素影响：（1）附着在单根血影蛋白细丝上的带3分子数量，以及（2）肌动蛋白连接点的磷酸化。血影蛋白柔韧性变化的速率取决于带3分子的重排。
