Kitisripanya Tharita, Jutathis Kamonthip, Inyai Chadathorn, Komaikul Jukrapun, Udomsin Orapin, Yusakul Gorawit, Tanaka Hiroyuki, Putalun Waraporn
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand.
J Nat Med. 2016 Apr;70(2):296-9. doi: 10.1007/s11418-015-0949-x. Epub 2015 Nov 13.
Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.
免疫原质量是影响抗体特性的一个重要因素。针对美豆雌醇的高特异性抗体可用于开发葛属产品的质量控制免疫分析方法。在本研究中，我们研究了不同的免疫原制备方法如何影响抗体特性。结果表明，与用高碘酸盐反应制备的免疫原相比，用曼尼希反应制备的免疫原能产生对美豆雌醇具有更高特异性和灵敏度的抗体。结果表明，曼尼希反应保持了美豆雌醇的原始结构，并产生了用于开发免疫分析的有用抗体。
