Wang Du, Zhang Zhaowei, Li Peiwu, Zhang Qi, Ding Xiaoxia, Zhang Wen
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences , Wuhan 430062, China.
J Agric Food Chem. 2015 Dec 2;63(47):10313-8. doi: 10.1021/acs.jafc.5b03746. Epub 2015 Nov 19.
Immunochromatographic (IC) assays are considered suitable diagnostic tools for the determination of mycotoxins. A europium nanospheres-based time-resolved fluorescence immunoassay (Eu-Nano-TRFIA), based on a monoclonal antibody and a portable TRFIA reader, was developed to determine total aflatoxin (including aflatoxins B1, B2, G1, and G2) levels in feed samples. Under optimized conditions, the Eu-Nano-TRFIA method detected total aflatoxin within 12 min. It showed good linearity (R(2) > 0.985), LOD of 0.16 μg/kg, a wide dynamic range of 0.48-30.0 μg/kg, recovery rates of 83.9-113.9%, and coefficients of variation (CVs) of 3.5-8.8%. In the 397 samples from company and livestock farms throughout China, the detection rate was 78.3%, concentrations were 0.50-145.30 μg/kg, the highest total aflatoxin content was found in cottonseed meal, and corn was found to be the most commonly contaminated feed. This method could be a powerful alternative for the rapid and ultrasensitive determination of total aflatoxin in quality control and meet the required Chinese maximum residue limits.
免疫层析（IC）检测法被认为是测定霉菌毒素的合适诊断工具。基于单克隆抗体和便携式时间分辨荧光免疫分析（TRFIA）读数仪，开发了一种基于铕纳米球的时间分辨荧光免疫分析方法（Eu-Nano-TRFIA），用于测定饲料样品中的总黄曲霉毒素（包括黄曲霉毒素B1、B2、G1和G2）水平。在优化条件下，Eu-Nano-TRFIA方法在12分钟内检测出总黄曲霉毒素。该方法具有良好的线性（R(2) > 0.985），检测限为0.16 μg/kg，动态范围宽，为0.48 - 30.0 μg/kg，回收率为83.9 - 113.9%，变异系数（CVs）为3.5 - 8.8%。在来自中国各地公司和养殖场的397份样品中，检出率为78.3%，浓度为0.50 - 145.30 μg/kg，棉籽粕中总黄曲霉毒素含量最高，玉米是最常被污染的饲料。该方法可为质量控制中快速、超灵敏测定总黄曲霉毒素提供有力的替代方法，并符合中国规定的最大残留限量要求。
